Tatiana Segura
Biomedical Engineering
Professor of Biomedical Engineering
Research Themes
Biomaterials, Drug & Gene Delivery, Tissue Engineering & Regenerative Medicine
Research Interests
The design of biomaterials to promote endogenous repair and reducing inflammation through the design of the geometry of the material, and delivering genes, proteins and drugs.
Bio
Tatiana Segura is a Professor of Biomedical Engineering, Neurology, and Dermatology at Duke University. She received her B.S. degree in Bioengineering from the University of California, Berkeley (UC Berkeley) and her doctorate in Chemical Engineering from Northwestern University. She began her career in Biomaterials research during her doctoral work working with Prof. Lonnie Shea. She designed hydrogels for local non-viral gene delivery, a topic that she still works on today. She continued her Biomaterials training during her postdoctoral work with Jeffrey Hubbell. There she worked on the design of hydrogels and self-assembled polysulfides for gene delivery. She began her independent career at the University of California, Los Angeles (UCLA) in the Department of Chemical and Biomolecular Engineering reaching the title of Professor. At UCLA she participated actively in service culminating with her election as department Vice Chair and running the Graduate Program. At Duke she has continued to be heavily involved in service at the department, school, and university level. In only 5 years, she has Chaired 6 committees, and participated in at least 6 more, is the direct mentor to two young assistant professors, is the Co-director of the Center for Biotechnology and Tissue Engineering and serves as MPI of the T32 Biotechnology Training grant. Notably she is currently the Chair of the BME department Diversity Equity and Inclusion Committee.
Prof. Segura’s research is centered on biomaterials and in engineering biomaterial-soft tissue interactions to promote repair and regeneration. Together with her lab members, she designs new biomaterial interventions that can promote brain plasticity after stroke, promote scarless healing in skin wounds, induce tolerance of transplanted skin, and promote constructive immune responses after biomaterial implantation. Currently, her lab has 12 graduate students, 4 postdoctoral scholars, 2 master students, 1 plastic surgery resident, 16 undergraduate students, one high school student, and one research associate.
Professor Segura has received numerous awards and distinctions during her career, including being named a Senior Member of the National Academy of Inventors, receiving the Acta Biomaterialia Silver Medal, a CAREER Award from the National Science Foundation, a Outstanding Young Investigator Award from the American Society of Gene and Cell Therapy, and a National Scientist Development Grant from the American Heart Association. She was also named a Fellow of the American Institute for Medical and Biological Engineers (AIMBE). Professor Segura has published over 100 peer-reviewed papers and reviews and has over 10,000 citations. Her laboratory has been continuously funded since 2008 with several grants from the National Institutes of Health (NIH).
Education
- B.S. University of California, Berkeley, 1999
- Ph.D. Northwestern University, 2004
Positions
- Professor of Biomedical Engineering
- Professor in Neurology
- Professor in Dermatology
- Affiliate of the Duke Regeneration Center
Awards, Honors, and Distinctions
- Clemson Award for Contributions to Literature. World Biomaterials Conference. 2024
- Senior Member. National Academy of Inventors. 2023
- Silver Medal. Acta Biomaterialia. 2021
- 15 d/e Plenary Award. AICHE Food, Pharmaceutical, and Bioengineering Division. 2018
- College of Fellows. American Institute for Medical and Biological Engineering. 2016
- Outstanding Young Investigator Award. American Society of Gene and Cell Therapy. 2009
- National Scientist Development Grant. American Heart Association. 2009
- CAREER Award. National Science Foundation. 2008
- Exploratory Research Grant Award R21. NIBIB. 2008
- F32 Postdoctoral NRSA Fellowship. National Institutes of Health. 2004
- Dissertation Henderson Fellowship. Northwestern University. 2003
- NIH Predoctoral Biotechnology Training Grant. Northwestern University. 2000
Courses Taught
- NEUROSCI 495: Research Independent Study 3
- NEUROSCI 494: Research Independent Study 2
- NEUROSCI 493: Research Independent Study 1
- EGR 491: Projects in Engineering
- EGR 393: Research Projects in Engineering
- BME 792: Continuation of Graduate Independent Study
- BME 791: Graduate Independent Study
- BME 789: Internship in Biomedical Engineering
- BME 771: Bioconjugation in drug biomaterials and drug delivery systems
- BME 494: Projects in Biomedical Engineering (GE)
- BME 493: Projects in Biomedical Engineering (GE)
- BME 394: Projects in Biomedical Engineering (GE)
Publications
- Segura T. From Soft Microgel Assemblies to Advanced Healthcare Materials. Advanced healthcare materials. 2024 Oct;13(25):e2402905.
- Wilson KL, Joseph NI, Onweller LA, Anderson AR, Darling NJ, David-Bercholz J, et al. SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis after Stroke. Advanced healthcare materials. 2024 Oct;13(25):e2302081.
- Kurt E, Devlin G, Asokan A, Segura T. Gene Delivery From Granular Scaffolds for Tunable Biologics Manufacturing. Small. 2024 Aug;20(31):e2309911.
- Wang MF, Ouyang Y, Segura T, Muddiman DC. Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain. Analytical and bioanalytical chemistry. 2024 Jul;416(18):4207–18.
- Curvino EJ, Roe EF, Freire Haddad H, Anderson AR, Woodruff ME, Votaw NL, et al. Engaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibres. Nat Biomed Eng. 2024 May;8(5):628–49.
- Liu Y, Suarez-Arnedo A, Caston ELP, Riley L, Schneider M, Segura T. Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds. Advanced materials (Deerfield Beach, Fla). 2023 Dec;35(49):e2304049.
- Riley L, Cheng P, Segura T. Identification and analysis of 3D pores in packed particulate materials. Nature Computational Science. 2023 Nov 21;3(11):975–92.
- Phan NV, Rathbun EM, Ouyang Y, Carmichael ST, Segura T. Biology-driven material design for ischaemic stroke repair. Nature Reviews Bioengineering. 2023 Oct 27;
- Liu Y, Suarez-Arnedo A, Riley L, Miley T, Xia J, Segura T. Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds. Advanced healthcare materials. 2023 Oct;12(26):e2300823.
- Riley L, Wei G, Bao Y, Cheng P, Wilson KL, Liu Y, et al. Void Volume Fraction of Granular Scaffolds. Small (Weinheim an der Bergstrasse, Germany). 2023 Oct;19(40):e2303466.
- Wilson KL, Onweller LA, Joseph NI, David-Bercholz J, Darling NJ, Segura T. SDF-1 Bound Heparin Nanoparticles Recruit Progenitor Cells for Their Differentiation and Promotion of Angiogenesis After Stroke. bioRxiv. 2023 Jul 5;
- Samal J, Palomino TV, Chen J, Muddiman DC, Segura T. Enhanced Detection of Charged N-Glycans in the Brain by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization Mass Spectrometric Imaging. Analytical chemistry. 2023 Jul;95(29):10913–20.
- Wang MF, Sohn AL, Samal J, Erning K, Segura T, Muddiman DC. Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI. Journal of the American Society for Mass Spectrometry. 2023 May;34(5):869–77.
- Liu Y, Suarez-Arnedo A, Shetty S, Wu Y, Schneider M, Collier JH, et al. A Balance between Pro-Inflammatory and Pro-Reparative Macrophages is Observed in Regenerative D-MAPS. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2023 Apr;10(11):e2204882.
- Wang Y, Wang C, Sylvers J, Segura T, Yuan F. Nanoenhancer for improving naked DNA electrotransfection In vivo. Frontiers in bioengineering and biotechnology. 2023 Jan;11:1181795.
- Swan SL, Mehta N, Ilich E, Shen SH, Wilkinson DS, Anderson AR, et al. IL7 and IL7 Flt3L co-expressing CAR T cells improve therapeutic efficacy in mouse EGFRvIII heterogeneous glioblastoma. Front Immunol. 2023;14:1085547.
- Kurt E, Segura T. Gene Delivery from Flowable Linked Irregular Particle (FLIP) Scaffolds for Advanced Biologics Manufacturing. In: MOLECULAR THERAPY. 2023. p. 214–5.
- Anderson AR, Segura T. Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture. Journal of visualized experiments : JoVE. 2022 Oct;(188).
- Anderson AR, Nicklow E, Segura T. Particle fraction is a bioactive cue in granular scaffolds. Acta biomaterialia. 2022 Sep;150:111–27.
- Sideris E, Kioulaphides S, Wilson K, Yu A, Chen J, Carmichael ST, et al. Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke. Advanced therapeutics. 2022 Aug;5(8):2200048.
- Wilson KL, Pérez SCL, Naffaa MM, Kelly SH, Segura T. Stoichiometric Post-Modification of Hydrogel Microparticles Dictates Neural Stem Cell Fate in Microporous Annealed Particle Scaffolds. Advanced materials (Deerfield Beach, Fla). 2022 Aug;34(33):e2201921.
- Kurt E, Segura T. Nucleic Acid Delivery from Granular Hydrogels. Advanced healthcare materials. 2022 Feb;11(3):e2101867.
- Xin S, Zhang L, Phan N, Carmichael T, Segura T. Reactive astrocyte derived extracellular vesicles promote functional repair post stroke. bioRxiv. 2022.
- Liu Y, Suarez-Arnedo A, Shetty S, Wu Y, Schneider M, Collier J, et al. A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPS. bioRxiv. 2022.
- Liu Y, Suarez-Arnedo A, Riley L, Miley T, Xia J, Segura T. Spatial confinement modulates macrophage response in microporous annealed particle (MAP) scaffolds. bioRxiv. 2022.
- Riley L, Wei G, Bao Y, Cheng P, Wilson K, Liu Y, et al. Void volume fraction of granular scaffolds. bioRxiv. 2022.
- Samal J, Segura T. Injectable biomaterial shuttles for cell therapy in stroke. Brain research bulletin. 2021 Nov;176:25–42.
- Xi W, Hegde V, Zoller SD, Park HY, Hart CM, Kondo T, et al. Point-of-care antimicrobial coating protects orthopaedic implants from bacterial challenge. Nature communications. 2021 Sep;12(1):5473.
- Griffin DR, Archang MM, Kuan C-H, Weaver WM, Weinstein JS, Feng AC, et al. Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing. Nature materials. 2021 Apr;20(4):560–9.
- Escuin-Ordinas H, Liu Y, Sun L, Hugo W, Dimatteo R, Huang RR, et al. Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects. PloS one. 2021 Jan;16(6):e0252597.
- Anderson AR, Segura T. Injectable biomaterials for treatment of glioblastoma. Advanced materials interfaces. 2020 Oct;7(20):2001055.
- Wilson KL, Carmichael ST, Segura T. Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. Journal of visualized experiments : JoVE. 2020 Oct;(164).
- McMillan AH, Thomée EK, Dellaquila A, Nassman H, Segura T, Lesher-Pérez SC. Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices. Micromachines. 2020 Jul;11(8):E731.
- Erning K, Segura T. Materials to Promote Recovery After Stroke. Current opinion in biomedical engineering. 2020 Jun;14:9–17.
- Darling NJ, Xi W, Sideris E, Anderson AR, Pong C, Carmichael ST, et al. Click by Click Microporous Annealed Particle (MAP) Scaffolds. Advanced healthcare materials. 2020 May;9(10):e1901391.
- Hegde V, Park HY, Dworsky E, Zoller SD, Xi W, Johansen DO, et al. The Use of a Novel Antimicrobial Implant Coating In Vivo to Prevent Spinal Implant Infection. Spine. 2020 Mar;45(6):E305–11.
- Griffin D, Archang M, Kuan C, Weaver W, Weinstein J, Feng AC, et al. Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing. bioRxiv. 2020.
- Daly AC, Riley L, Segura T, Burdick JA. Hydrogel microparticles for biomedical applications. Nature reviews Materials. 2020 Jan;5(1):20–43.
- Liu Y, Segura T. Biomaterials-Mediated Regulation of Macrophage Cell Fate. Frontiers in bioengineering and biotechnology. 2020 Jan;8:609297.
- Riley L, Schirmer L, Segura T. Granular hydrogels: emergent properties of jammed hydrogel microparticles and their applications in tissue repair and regeneration. Current opinion in biotechnology. 2019 Dec;60:1–8.
- Koh J, Griffin DR, Archang MM, Feng A-C, Horn T, Margolis M, et al. Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds. Small (Weinheim an der Bergstrasse, Germany). 2019 Sep;15(39):e1903147.
- Stefanelli VL, Choudhury S, Hu P, Liu Y, Schwenzer A, Yeh C-R, et al. Citrullination of fibronectin alters integrin clustering and focal adhesion stability promoting stromal cell invasion. Matrix biology : journal of the International Society for Matrix Biology. 2019 Sep;82:86–104.
- Truong NF, Kurt E, Tahmizyan N, Lesher-Pérez SC, Chen M, Darling NJ, et al. Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer. Acta biomaterialia. 2019 Aug;94:160–72.
- Truong NF, Lesher-Pérez SC, Kurt E, Segura T. Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds. Bioconjugate chemistry. 2019 Feb;30(2):476–86.
- Pannier AK, Kozisek T, Segura T. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles. Methods in molecular biology (Clifton, NJ). 2019 Jan;1943:177–97.
- Stavrakis AI, Zhu S, Loftin AH, Weixian X, Niska J, Hegde V, et al. Controlled Release of Vancomycin and Tigecycline from an Orthopaedic Implant Coating Prevents Staphylococcus aureus Infection in an Open Fracture Animal Model. BioMed research international. 2019 Jan;2019:1638508.
- Darling N, Xi W, Sideris E, Anderson A, Pong C, Carmichael T, et al. Click by Click Microporous Annealed Particle (MAP) Scaffolds. bioRxiv. 2019.
- Sideris E, Yu A, Chen J, Carmichael T, Segura T. Hyaluronic acid particle hydrogels decrease cerebral atrophy and promote pro-reparative astrocyte/axonal infiltration in the core after ischemic stroke. bioRxiv. 2019.
- Darling N, Sideris E, Hamada N, Segura T. Injectable and spatially patterned microporous annealed particle (MAP) hydrogels for tissue repair applications. Advanced Science. 2018 Oct 11;1–8.
- Youngblood RL, Truong NF, Segura T, Shea LD. It's All in the Delivery: Designing Hydrogels for Cell and Non-viral Gene Therapies. Molecular therapy : the journal of the American Society of Gene Therapy. 2018 Sep;26(9):2087–106.
- Villate-Beitia I, Truong NF, Gallego I, Zárate J, Puras G, Pedraz JL, et al. Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery. RSC advances. 2018 Sep;8(56):31934–42.
- Nih LR, Gojgini S, Carmichael ST, Segura T. Dual-function injectable angiogenic biomaterial for the repair of brain tissue following stroke. Nature materials. 2018 Jul;17(7):642–51.
- Zhu S, Li S, Escuin-Ordinas H, Dimatteo R, Xi W, Ribas A, et al. Accelerated wound healing by injectable star poly(ethylene glycol)-b-poly(propylene sulfide) scaffolds loaded with poorly water-soluble drugs. Journal of controlled release : official journal of the Controlled Release Society. 2018 Jul;282:156–65.
- Dimatteo R, Darling NJ, Segura T. In situ forming injectable hydrogels for drug delivery and wound repair. Advanced drug delivery reviews. 2018 Mar;127:167–84.
- Truong NF, Segura T. Sustained Transgene Expression via Hydrogel-Mediated Gene Transfer Results from Multiple Transfection Events. ACS biomaterials science & engineering. 2018 Mar;4(3):981–7.
- Fei P, Nie J, Lee J, Ding Y, Li S, Zhang H, et al. Sub-voxel light-sheet microscopy for high-resolution, high-throughput volumetric imaging of large biomedical specimens. bioRxiv. 2018.
- Nih LR, Carmichael ST, Segura T. Pro-Angiogenic Regenerative Therapies for the Damaged Brain: A Tissue Engineering Approach. In: Biological and Medical Physics, Biomedical Engineering. Springer International Publishing; 2018. p. 177–87.
- Griffin US. CONTROLLABLE SELF-ANNEALING MICROGEL PARTICLES FOR BIOMEDICAL APPLICATIONS. 2017.
- Li S, Nih LR, Bachman H, Fei P, Li Y, Nam E, et al. Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nature Materials. 2017 Sep 1;16(9):953–61.
- Ray A, Li S, Segura T, Ozcan A. High-throughput quantification of nanoparticle degradation using computational microscopy and its application to drug delivery nanocapsules. ACS Photonics. 2017;4:1216–24.
- Zhu X, Gojgini S, Chen T-H, Fei P, Dong S, Ho C-M, et al. Directing three-dimensional multicellular morphogenesis by self-organization of vascular mesenchymal cells in hyaluronic acid hydrogels. Journal of biological engineering. 2017;11:12–12.
- Nih LR, Moshayedi P, Llorente IL, Berg AR, Cinkornpumin J, Lowry WE, et al. Engineered HA hydrogel for stem cell transplantation in the brain: Biocompatibility data using a design of experiment approach. Data in brief. 2017;10:202–9.
- Nih LR, Sideris E, Carmichael ST, Segura T. Injection of Microporous Annealing Particle (MAP) Hydrogels in the Stroke Cavity Reduces Gliosis and Inflammation and Promotes NPC Migration to the Lesion. Advanced Materials. 2017;29.
- Ding Y, Abiri A, Abiri P, Li S, Chang C-C, Baek KI, et al. Integrating light-sheet imaging with virtual reality to recapitulate developmental cardiac mechanics. JCI insight. 2017;2.
- Segura T, Hubbell JA. Triblock copolymers for cytoplasmic delivery of gene-based drugs. 2016.
- Griffin DR, Weaver W, Segura T, Di Carlo D, Scumpia P. Controllable self-annealing microgel particles for biomedical applications. 2016.
- Nih LR, Carmichael ST, Segura T. Hydrogels for brain repair after stroke: an emerging treatment option. Current opinion in biotechnology. 2016;40:155–63.
- Zhu S, Segura T. Cell-Demanded VEGF Release via Nanocapsules Elicits Different Receptor Activation Dynamics and Enhanced Angiogenesis. Annals of biomedical engineering. 2016;44:1983–92.
- Li J, Zhang L, Liu Y, Wen J, Wu D, Xu D, et al. An intracellular protein delivery platform based on glutathione-responsive protein nanocapsules. Chemical Communications. 2016;52:13608–11.
- Sideris E, Griffin DR, Ding Y, Li S, Weaver WM, Di Carlo D, et al. Particle hydrogels based on hyaluronic acid building blocks. ACS Biomaterials Science & Engineering. 2016;2:2034–41.
- Darling NJ, Hung Y-S, Sharma S, Segura T. Controlling the kinetics of thiol-maleimide Michael-type addition gelation kinetics for the generation of homogenous poly (ethylene glycol) hydrogels. Biomaterials. 2016;101:199–206.
- Zhu X, Gojgini S, Chen T-H, Teng F, Fei P, Dong S, et al. Three dimensional tubular structure self-assembled by vascular mesenchymal cells at stiffness interfaces of hydrogels. Biomedicine & Pharmacotherapy. 2016;83:1203–11.
- Escuin-Ordinas H, Li S, Xie MW, Sun L, Hugo W, Huang RR, et al. Cutaneous wound healing through paradoxical MAPK activation by BRAF inhibitors. Nature communications. 2016;7.
- Stavrakis AI, Zhu S, Hegde V, Loftin AH, Ashbaugh AG, Niska JA, et al. In Vivo Efficacy of a ´93Smart´94 Antimicrobial Implant Coating. JBJS. 2016;98:1183–9.
- Moshayedi P, Nih LR, Llorente IL, Berg AR, Cinkornpumin J, Lowry WE, et al. Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain. Biomaterials. 2016;105:145–55.
- Lesher-Perez SC, Segura T, Moraes C. Getting there is half the battle: recent advances in delivering therapeutics. Integrative Biology. 2016;8:8–11.
- Lu Y, Segura T, Zhu S, Wen J. Enzyme responsive nanocapsules for protein delivery. 2015.
- Maynard HD, Segura T, Grover G, Lam J. Method of creating hydrogels through oxime bond fomration. 2015.
- Segura T. Enzyme-assisted spatial decoration of biomaterials. 2015.
- Du J, Jin J, Liu Y, Li J, Tokatlian T, Lu Z, et al. Erratum: Gold-nanocrystal-enhanced bioluminescent nanocapsules (ACS Nano (2014) 8 (9964-9969) DOI: 10.1021/nn504371h). ACS Nano. 2015 Feb 24;9(2):2213.
- Cam C, Zhu S, Truong NF, Scumpia PO, Segura T. Systematic evaluation of natural scaffolds in cutaneous wound healing. Journal of Materials Chemistry B. 2015 Jan 1;3(40):7986–92.
- Lam J, Carmichael ST, Lowry WE, Segura T. Hydrogel Design of Experiments Methodology to Optimize Hydrogel for iPSC-NPC Culture. Advanced healthcare materials. 2015;4:534–9.
- Du J, Jin J, Liu Y, Li J, Tokatlian T, Lu Z, et al. Correction to Gold-Nanocrystal-Enhanced Bioluminescent Nanocapsules. ACS nano. 2015;9:2213–2213.
- Tokatlian T, Cam C, Segura T. Porous hyaluronic acid hydrogels for localized nonviral DNA delivery in a diabetic wound healing model. Advanced healthcare materials. 2015;4:1084–91.
- Zhu S, Nih L, Carmichael ST, Lu Y, Segura T. Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control. Advanced Materials. 2015;27:3620–5.
- Griffin DR, Weaver WM, Scumpia PO, Di Carlo D, Segura T. Accelerated wound healing by injectable microporous gel scaffolds assembled from annealed building blocks. Nature materials. 2015;14:737–44.
- Boehnke N, Cam C, Bat E, Segura T, Maynard HD. Imine hydrogels with tunable degradability for tissue engineering. Biomacromolecules. 2015;16:2101–8.
- Siegman S, Truong NF, Segura T. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia. 2015;28:45–54.
- Konstantopoulos K, Segura T. Editorial overview: Biological engineering: Advances in smart and controlled delivery of therapeutic molecules. Elsevier; 2015.
- Griffin DR, Borrajo J, Soon A, Acosta-Vélez GF, Oshita V, Darling N, et al. Erratum: Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation (ChemBioChem (2014) 15 (233-242) DOI: 10.1002/cbic.201300687). ChemBioChem. 2014 Mar 3;15(4):485–6.
- Cam C, Segura T. Chemical sintering generates uniform porous hyaluronic acid hydrogels. Acta biomaterialia. 2014;10:205–13.
- Tokatlian T, Cam C, Segura T. Non-viral DNA delivery from porous hyaluronic acid hydrogels in mice. Biomaterials. 2014;35:825–35.
- Lam J, Truong NF, Segura T. Design of cell–matrix interactions in hyaluronic acid hydrogel scaffolds. Acta biomaterialia. 2014;10:1571–80.
- Griffin DR, Borrajo J, Soon A, Acosta-Vèlez GF, Oshita V, Darling N, et al. Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation. ChemBioChem. 2014;15:233–42.
- Zhu S, Segura T. Hydrogel-based nanocomposites of therapeutic proteins for tissue repair. Current opinion in chemical engineering. 2014;4:128–36.
- Nowak-Sliwinska P, Segura T, Iruela-Arispe ML. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis. 2014;17:779–804.
- Lam J, Lowry WE, Carmichael ST, Segura T. Delivery of iPS-NPCs to the Stroke Cavity within a Hyaluronic Acid Matrix Promotes the Differentiation of Transplanted Cells. Advanced functional materials. 2014;24:7053–62.
- Du J, Jin J, Liu Y, Li J, Tokatlian T, Lu Z, et al. Gold-nanocrystal-enhanced bioluminescent nanocapsules. ACS nano. 2014;8:9964–9.
- Lam J, Lowry B, Carmichael ST, Segura T. Optimizing hydrogels in vitro for transplantation of iPS-NPCs in vivo after stroke. 2014;
- Lam J, Segura T. The modulation of MSC integrin expression by RGD presentation. Biomaterials. 2013;34:3938–47.
- Cam C, Segura T. Matrix-based gene delivery for tissue repair. Current opinion in biotechnology. 2013;24:855–63.
- Dhaliwal A, Oshita V, Segura T. Transfection in the third dimension. Integrative Biology. 2013;5:1206–16.
- Pannier AK, Segura T. Surface-and hydrogel-mediated delivery of nucleic acid nanoparticles. Nanotechnology for Nucleic Acid Delivery: Methods and Protocols. 2013;149–69.
- Segura T, Ng Q. Multivalent clustering targeting strategy for drug carriers. 2012.
- Dhaliwal A, Lam J, Maldonado M, Lin C, Segura T. Extracellular matrix modulates non-viral gene transfer to mouse mesenchymal stem cells. Soft Matter. 2012 Feb 7;8(5):1451–9.
- Grover GN, Lam J, Nguyen TH, Segura T, Maynard HD. Biocompatible hydrogels by oxime Click chemistry. Biomacromolecules. 2012;13:3013–7.
- Dhaliwal A, Maldonado M, Lin C, Segura T. Cellular cytoskeleton dynamics modulates non-viral gene delivery through RhoGTPases. PloS one. 2012;7:e35046–e35046.
- Tokatlian T, Cam C, Siegman SN, Lei Y, Segura T. Design and characterization of microporous hyaluronic acid hydrogels for in vitro gene transfer to mMSCs. Acta biomaterialia. 2012;8:3921–31.
- Zhang J, Lei Y, Dhaliwal A, Ng QKT, Du J, Yan M, et al. Protein- Polymer Nanoparticles for Nonviral Gene Delivery. Biomacromolecules. 2011;12:1006–14.
- Zhang J, Tokatlian T, Zhong J, Ng QKT, Patterson M, Lowry WE, et al. Physically Associated Synthetic Hydrogels with Long-Term Covalent Stabilization for Cell Culture and Stem Cell Transplantation. Advanced Materials. 2011;23:5098–103.
- Lei Y, Gojgini S, Lam J, Segura T. The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels. Biomaterials. 2011;32:39–47.
- Collier JH, Segura T. Evolving the use of peptides as components of biomaterials. Biomaterials. 2011;32:4198–204.
- Lei Y, Rahim M, Ng Q, Segura T. Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery. Journal of controlled release. 2011;153:255–61.
- Anderson SM, Siegman SN, Segura T. The effect of vascular endothelial growth factor (VEGF) presentation within fibrin matrices on endothelial cell branching. Biomaterials. 2011;32:7432–43.
- Gojgini S, Tokatlian T, Segura T. Utilizing cell–matrix interactions to modulate gene transfer to stem cells inside hyaluronic acid hydrogels. Molecular pharmaceutics. 2011;8:1582–91.
- Wen J, Anderson SM, Du J, Yan M, Wang J, Shen M, et al. Controlled Protein Delivery Based on Enzyme-Responsive Nanocapsules. Advanced materials. 2011;23:4549–53.
- Anderson SM, Shergill B, Barry ZT, Manousiouthakis E, Chen TT, Botvinick E, et al. VEGF internalization is not required for VEGFR-2 phosphorylation in bioengineered surfaces with covalently linked VEGF. Integrative biology. 2011;3:887–96.
- Ng QKT, Su H, Armijo AL, Czernin J, Radu CG, Segura T. Clustered Arg–Gly–Asp peptides enhances tumor targeting of nonviral vectors. ChemMedChem. 2011;6:623–7.
- Zhang J, Du J, Yan M, Dhaliwal A, Wen J, Liu F, et al. Synthesis of protein nano-conjugates for cancer therapy. Nano Research. 2011;4:425–33.
- Tokatlian T, Segura T. siRNA applications in nanomedicine. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2010;2:305–15.
- Tokatlian T, Shrum CT, Kadoya WM, Segura T. Protease degradable tethers for controlled and cell-mediated release of nanoparticles in 2-and 3-dimensions. Biomaterials. 2010;31:8072–80.
- Lei Y, Huang S, Sharif-Kashani P, Chen Y, Kavehpour P, Segura T. Incorporation of active DNA/cationic polymer polyplexes into hydrogel scaffolds. Biomaterials. 2010;31:9106–16.
- Lei Y, Ng QKT, Segura T. Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffolds. Microscopy research and technique. 2010;73:910–7.
- Chen TT, Luque A, Lee S, Anderson SM, Segura T, Iruela-Arispe ML. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. The Journal of cell biology. 2010;188:595–609.
- Yan M, Du J, Gu Z, Liang M, Hu Y, Zhang W, et al. A novel intracellular protein delivery platform based on single-protein nanocapsules. Nature nanotechnology. 2010;5:48–53.
- Dhaliwal A, Maldonado M, Han Z, Segura T. Differential uptake of DNA–poly (ethylenimine) polyplexes in cells cultured on collagen and fibronectin surfaces. Acta biomaterialia. 2010;6:3436–47.
- Du J, Yu C, Pan D, Li J, Chen W, Yan M, et al. Quantum-dot-decorated robust transductable bioluminescent nanocapsules. Journal of the American Chemical Society. 2010;132:12780–1.
- Lei Y, Segura T. DNA delivery from matrix metalloproteinase degradable poly (ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells. Biomaterials. 2009;30:254–65.
- Anderson SM, Chen TT, Iruela-Arispe ML, Segura T. The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF. Biomaterials. 2009;30:4618–28.
- Ng QKT, Sutton MK, Soonsawad P, Xing L, Cheng H, Segura T. Engineering clustered ligand binding into nonviral vectors: αvβ3 targeting as an example. Molecular Therapy. 2009;17:828–36.
- Adel ddotow C, Segura T, Hubbell JA, Frey P. The effect of enzymatically degradable poly (ethylene glycol) hydrogels on smooth muscle cell phenotype. Biomaterials. 2008;29:314–26.
- Segura T, Hubbell JA. Synthesis and in vitro characterization of an ABC triblock copolymer for siRNA delivery. Bioconjugate chemistry. 2007;18:736–45.
- Segura T. Formulations and Delivery Limitations of Nucleic-Acid-Based Therapies. Handbook of Pharmaceutical Biotechnology. 2007;1013–59.
- Segura T. Materials for cell-triggered and efficient gene transfer. Nanomedicine: Nanotechnology, Biology and Medicine. 2007;3:344–344.
- Segura T, Schmokel H, Hubbell JA. RNA interference targeting hypoxia inducible factor 1α reduces post-operative adhesions in rats. Journal of Surgical Research. 2007;141:162–70.
- Segura T, Shea LD, Pannier AK, Bengali Z, Jang J-H, Chung P, et al. Controlled surface-associated delivery of genes and oligonucleotides. Biological Systems Engineering: Papers and Publications. 2006. p. 145–145.
- Segura T, Shea LD. Controlled surface-associated delivery of genes and oligonucleotides. 2005.
- Bengali Z, Pannier AK, Segura T, Anderson BC, Jang J-H, Mustoe TA, et al. Gene delivery through cell culture substrate adsorbed DNA complexes. Biotechnology and bioengineering. 2005;90:290–302.
- Segura T, Chung PH, Shea LD. DNA delivery from hyaluronic acid-collagen hydrogels via a substrate-mediated approach. Biomaterials. 2005;26:1575–84.
- Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD. Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern. Biomaterials. 2005;26:359–71.
- Segura T, Volk MJ, Shea LD. Substrate-mediated DNA delivery: role of the cationic polymer structure and extent of modification. Journal of Controlled Release. 2003;93:69–84.
- Segura T, Shea LD. Surface-tethered DNA complexes for enhanced gene delivery. Bioconjugate Chemistry. 2002;13:621–9.
- Segura T, Shea LD. Materials for non-viral gene delivery. Annual Review of Materials Research. 2001;31:25–46.
In The News
- Measuring Empty Spaces for Better Wound Healing (Dec 14, 2023 | Pratt School of Engineering)
- Tatiana Segura Named National Academy of Inventors Senior Member (Feb 20, 2023 | (Pratt School of Engineering))
- Segura Named Co-Director of Center for Biomolecular and Tissue Engineering (Feb 13, 2023 | (Pratt School of Engineering))
- A Gel That Regrows the Brain (Mar 2, 2022 | )
- Antimicrobial Coating for Orthopedic Implants Prevents Dangerous Infections (Sep 27, 2021 | Pratt School of Engineering)
- Duke Celebrates Women and Girls in Science Day (Feb 10, 2021 | )
- Wound-Healing Biomaterials Activate Immune System for Stronger Skin (Nov 11, 2020 | Pratt School of Engineering)
- No Ordinary Gel: New Tools to Help the Body Repair Brain and Muscle Tissue (Nov 5, 2019 | Pratt School of Engineering)
- New Biomaterial Helps Regrow Brain Tissue After Stroke in Mice (May 21, 2018 | Pratt School of Engineering)