Junjie Yao

Junjie Yao

Assistant Professor of Biomedical Engineering

Our mission at PI-Lab is to develop state-of-the-art photoacoustic tomography (PAT) technologies and translate PAT advances into diagnostic and therapeutic applications, especially in functional brain imaging and early cancer theranostics. PAT is the most sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, and is one of the fastest growing biomedical imaging technologies. Using numerous endogenous and exogenous contrasts, PAT can provide high-resolution images at scales covering organelles, cells, tissues, organs, small-animal organisms, up to humans, and can reveal tissue’s anatomical, functional, metabolic, and even histologic properties, with molecular and neuronal specificity.

At PI-Lab, we develop PAT technologies with novel and advanced imaging performance, in terms of spatial resolutions, imaging speed, penetration depth, detection sensitivity, and functionality. We are interested with all aspects of PAT technology innovations, including efficient light illumination, high-sensitivity ultrasonic detection, super-resolution PAT, high-speed imaging acquisition, novel PA genetic contrast, and precise image reconstruction. On top of the technological advancements, we are devoted to serve the broad life science and medical communities with matching PAT systems for various research and clinical needs. With its unique contrast mechanism, high scalability, and inherent functional and molecular imaging capabilities, PAT is well suited for a variety of pre-clinical applications, especially for studying tumor angiogenesis, cancer hypoxia, and brain disorders; it is also a promising tool for clinical applications in procedures such as cancer screening, melanoma staging, and endoscopic examination.

Appointments and Affiliations

  • Assistant Professor of Biomedical Engineering

Contact Information

  • Office Location: Hudson Hall Annex 261, Durham, NC 27708
  • Office Phone: (919) 681-0694
  • Email Address: junjie.yao@duke.edu
  • Websites:

Education

  • Ph.D. Washington University, 2012

Research Interests

Photoacoustic tomography (PAT) technologies, and translating PAT advances into diagnostic and therapeutic applications, especially in functional brain imaging and early cancer theranostics.

Awards, Honors, and Distinctions

  • Seno Medical Best Paper Award. SPIE conference Photons Plus Ultrasound 2016: Imaging and Sensing. 2016
  • Thousand Talents Program for Distinguished Chinese Young Scholar. Central Coordination Committee of China . 2016
  • Seno Medical Best Paper Award. SPIE conference Photons Plus Ultrasound 2015: Imaging and Sensing . 2015
  • Seno Medical Best Paper Award. SPIE conference Photons Plus Ultrasound 2013: Imaging and Sensing . 2013
  • Chinese Government Award for Outstanding Self-financed Students Aboard. The Education Ministry of China. 2012
  • Best Master Dissertation Award. Tsinghua University. 2008
  • Comprehensive Student Fellowship. Tsinghua University. 2008
  • KangShien Outstanding Graduate Fellowship. Tsinghua University. 2006
  • CyrusTang Fellowship (2002-2006). Tsinghua University. 2002

Courses Taught

  • BME 791: Graduate Independent Study

In the News

Representative Publications

  • Wang, LV; Yao, J, A practical guide to photoacoustic tomography in the life sciences., Nature Methods, vol 13 no. 8 (2016), pp. 627-638 [10.1038/nmeth.3925] [abs].
  • Zhou, Y; Yao, J; Wang, LV, Tutorial on photoacoustic tomography, Journal of Biomedical Optics, vol 21 no. 6 (2016), pp. 061007-061007 [10.1117/1.JBO.21.6.061007] [abs].
  • Hai, P; Yao, J; Li, G; Li, C; Wang, LV, Photoacoustic elastography, Optics Letters, vol 41 no. 4 (2016), pp. 725-725 [10.1364/OL.41.000725] [abs].
  • Yao, J; Kaberniuk, AA; Li, L; Shcherbakova, DM; Zhang, R; Wang, L; Li, G; Verkhusha, VV; Wang, LV, Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe., Nature Methods, vol 13 no. 1 (2016), pp. 67-73 [10.1038/nmeth.3656] [abs].
  • Lin, L; Yao, J; Li, L; Wang, LV, In vivo photoacoustic tomography of myoglobin oxygen saturation, Journal of Biomedical Optics, vol 21 no. 6 (2015), pp. 061002-061002 [10.1117/1.JBO.21.6.061002] [abs].
  • Yao, J; Wang, L; Yang, JM; Maslov, KI; Wong, TT; Li, L; Huang, CH; Zou, J; Wang, LV, High-speed label-free functional photoacoustic microscopy of mouse brain in action., Nature Methods, vol 12 no. 5 (2015), pp. 407-410 [10.1038/nmeth.3336] [abs].
  • Yao, J; Song, L; Wang, LV, Photoacoustic microscopy: superdepth, superresolution, and superb contrast., IEEE Pulse, vol 6 no. 3 (2015), pp. 34-37 [10.1109/mpul.2015.2409100] [abs].
  • Xia, J; Yao, J; Wang, LV, Photoacoustic tomography: principles and advances., Progress in Electromagnetics Research, vol 147 (2014), pp. 1-22 [abs].
  • Wang, L; Yao, J; Maslov, KI; Xing, W; Wang, LV, Ultrasound-heated photoacoustic flowmetry., Journal of Biomedical Optics, vol 18 no. 11 (2013) [10.1117/1.jbo.18.11.117003] [abs].
  • Yao, J; Wang, LV, Photoacoustic Microscopy., Laser & Photonics Reviews, vol 7 no. 5 (2013) [10.1002/lpor.201200060] [abs].
  • Zhang, R; Yao, J; Maslov, KI; Wang, LV, Structured-illumination photoacoustic Doppler flowmetry of axial flow in homogeneous scattering media., Applied Physics Letters, vol 103 no. 9 (2013) [10.1063/1.4819735] [abs].
  • Zhou, Y; Yao, J; Wang, LV, Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth., Optics Letters, vol 38 no. 14 (2013), pp. 2592-2595 [10.1364/ol.38.002592] [abs].
  • Rowland, KJ; Yao, J; Wang, L; Erwin, CR; Maslov, KI; Wang, LV; Warner, BW, Up-regulation of hypoxia-inducible factor 1 alpha and hemodynamic responses following massive small bowel resection., Journal of Pediatric Surgery, vol 48 no. 6 (2013), pp. 1330-1339 [10.1016/j.jpedsurg.2013.03.031] [abs].
  • Huang, C-H; Yao, J; Wang, LV; Zou, J, A water-immersible 2-axis scanning mirror microsystem for ultrasound andha photoacoustic microscopic imaging applications, Microsystem Technologies, vol 19 no. 4 (2013), pp. 577-582 [10.1007/s00542-012-1660-4] [abs].
  • Cai, X; Zhang, Y; Li, L; Choi, SW; MacEwan, MR; Yao, J; Kim, C; Xia, Y; Wang, LV, Investigation of neovascularization in three-dimensional porous scaffolds in vivo by a combination of multiscale photoacoustic microscopy and optical coherence tomography., Tissue Engineering, Part C, Methods, vol 19 no. 3 (2013), pp. 196-204 [10.1089/ten.tec.2012.0326] [abs].
  • Yao, J; Xia, J; Maslov, KI; Nasiriavanaki, M; Tsytsarev, V; Demchenko, AV; Wang, LV, Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo., NeuroImage, vol 64 (2013), pp. 257-266 [10.1016/j.neuroimage.2012.08.054] [abs].
  • Zhang, C; Maslov, K; Yao, J; Wang, LV, In vivo photoacoustic microscopy with 7.6-┬Ám axial resolution using a commercial 125-MHz ultrasonic transducer., Journal of Biomedical Optics, vol 17 no. 11 (2012) [10.1117/1.jbo.17.11.116016] [abs].
  • Yang, JM; Chen, R; Favazza, C; Yao, J; Li, C; Hu, Z; Zhou, Q; Shung, KK; Wang, LV, A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy., Optics express, vol 20 no. 21 (2012), pp. 23944-23953 [10.1364/oe.20.023944] [abs].
  • Yao, J; Huang, CH; Wang, L; Yang, JM; Gao, L; Maslov, KI; Zou, J; Wang, LV, Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror., Journal of Biomedical Optics, vol 17 no. 8 (2012), pp. 080505-080501 [10.1117/1.jbo.17.8.080505] [abs].
  • Yang, JM; Favazza, C; Chen, R; Yao, J; Cai, X; Maslov, K; Zhou, Q; Shung, KK; Wang, LV, Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo., Nature Medicine, vol 18 no. 8 (2012), pp. 1297-1302 [10.1038/nm.2823] [abs].
  • Yao, J; Maslov, KI; Wang, LV, In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism., Technology in cancer research & treatment, vol 11 no. 4 (2012), pp. 301-307 [10.7785/tcrt.2012.500278] [abs].
  • Rowland, KJ; Yao, J; Wang, L; Erwin, CR; Maslov, KI; Wang, LV; Warner, BW, Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy., Journal of Pediatric Surgery, vol 47 no. 6 (2012), pp. 1143-1149 [10.1016/j.jpedsurg.2012.03.020] [abs].
  • Krumholz, A; Wang, L; Yao, J; Wang, LV, Functional photoacoustic microscopy of diabetic vasculature., Journal of Biomedical Optics, vol 17 no. 6 (2012) [10.1117/1.jbo.17.6.060502] [abs].
  • Yao, J; Maslov, KI; Puckett, ER; Rowland, KJ; Warner, BW; Wang, LV, Double-illumination photoacoustic microscopy., Optics Letters, vol 37 no. 4 (2012), pp. 659-661 [10.1364/ol.37.000659] [abs].
  • Filonov, GS; Krumholz, A; Xia, J; Yao, J; Wang, LV; Verkhusha, VV, Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe., Angewandte Chemie International Edition, vol 51 no. 6 (2012), pp. 1448-1451 [10.1002/anie.201107026] [abs].
  • Tsytsarev, V; Maslov, KI; Yao, J; Parameswar, AR; Demchenko, AV; Wang, LV, In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog., Journal of Neuroscience Methods, vol 203 no. 1 (2012), pp. 136-140 [10.1016/j.jneumeth.2011.09.005] [abs].
  • Chatni, MR; Yao, J; Danielli, A; Favazza, CP; Maslov, KI; Wang, LV, Functional photoacoustic microscopy of pH., Journal of Biomedical Optics, vol 16 no. 10 (2011) [10.1117/1.3644495] [abs].
  • Yao, J; Wang, LV, Photoacoustic tomography: fundamentals, advances and prospects., Contrast Media & Molecular Imaging, vol 6 no. 5 (2011), pp. 332-345 [10.1002/cmmi.443] [abs].
  • Krumholz, A; Vanvickle-Chavez, SJ; Yao, J; Fleming, TP; Gillanders, WE; Wang, LV, Photoacoustic microscopy of tyrosinase reporter gene in vivo., Journal of Biomedical Optics, vol 16 no. 8 (2011) [10.1117/1.3606568] [abs].
  • Oladipupo, S; Hu, S; Kovalski, J; Yao, J; Santeford, A; Sohn, RE; Shohet, R; Maslov, K; Wang, LV; Arbeit, JM, VEGF is essential for hypoxia-inducible factor-mediated neovascularization but dispensable for endothelial sprouting., Proceedings of the National Academy of Sciences of USA, vol 108 no. 32 (2011), pp. 13264-13269 [10.1073/pnas.1101321108] [abs].
  • Tsytsarev, V; Hu, S; Yao, J; Maslov, K; Barbour, DL; Wang, LV, Photoacoustic microscopy of microvascular responses to cortical electrical stimulation., Journal of Biomedical Optics, vol 16 no. 7 (2011) [10.1117/1.3594785] [abs].
  • Yao, J; Maslov, KI; Zhang, Y; Xia, Y; Wang, LV, Label-free oxygen-metabolic photoacoustic microscopy in vivo., Journal of Biomedical Optics, vol 16 no. 7 (2011) [10.1117/1.3594786] [abs].
  • Oladipupo, SS; Hu, S; Santeford, AC; Yao, J; Kovalski, JR; Shohet, RV; Maslov, K; Wang, LV; Arbeit, JM, Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence., Blood, vol 117 no. 15 (2011), pp. 4142-4153 [10.1182/blood-2010-09-307538] [abs].
  • Wang, L; Maslov, K; Yao, J; Rao, B; Wang, LV, Fast voice-coil scanning optical-resolution photoacoustic microscopy., Optics Letters, vol 36 no. 2 (2011), pp. 139-141 [10.1364/ol.36.000139] [abs].
  • Kim, C; Song, HM; Cai, X; Yao, J; Wei, A; Wang, LV, In vivo photoacoustic mapping of lymphatic systems with plasmon-resonant nanostars., Journal of Materials Chemistry, vol 21 no. 9 (2011), pp. 2841-2844 [10.1039/c0jm04194g] [abs].
  • Yao, J; Maslov, KI; Shi, Y; Taber, LA; Wang, LV, In vivo photoacoustic imaging of transverse blood flow by using Doppler broadening of bandwidth., Optics Letters, vol 35 no. 9 (2010), pp. 1419-1421 [10.1364/ol.35.001419] [abs].
  • SONG, X-L; HU, G; HU, G; BAI, J, Three-Dimensional Reconstruction for Fluorescence Tomography Using Cylinder Phantoms, Ruan Jian Xue Bao/Journal of Software, vol 20 no. 5 (2010), pp. 1089-1095 [10.3724/SP.J.1001.2009.03560] [abs].
  • Yao, J; Wang, LV, Transverse flow imaging based on photoacoustic Doppler bandwidth broadening., Journal of Biomedical Optics, vol 15 no. 2 (2010) [10.1117/1.3339953] [abs].
  • Yao, J; Maslov, K; Hu, S; Wang, LV, Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo., Journal of Biomedical Optics, vol 14 no. 5 (2009) [10.1117/1.3251044] [abs].
  • Yao, JJ; Hu, G; Bai, J, Modeling and validation of light propagation in free-space for non-contact near-infrared fluorescent tomography, Hongwai Yu Haomibo Xuebao/Journal of Infrared and Millimeter Waves, vol 27 no. 5 (2008) [abs].
  • Yao, JJ; Hu, G; Yue, SH; Bai, J, A 3D-surface torso reconstruction method used in fluorescence molecular tomography of small animals, Zhongguo Shengwu Yixue Gongcheng Xuebao/Chinese Journal of Biomedical Engineering, vol 27 no. 3 (2008), pp. 360-365 [abs].
  • Hu, G; Yao, J; Bai, J, Full-angle optical imaging of near-infrared fluorescent probes implanted in small animals, Progress in Natural Science (Elsevier), vol 18 no. 6 (2008), pp. 707-711 [10.1016/j.pnsc.2007.11.021] [abs].