Junjie Yao

Associate Professor of Biomedical Engineering

Our mission at PI-Lab is to develop state-of-the-art photoacoustic tomography (PAT) technologies and translate PAT advances into diagnostic and therapeutic applications, especially in functional brain imaging and early cancer theranostics. PAT is the most sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, and is one of the fastest growing biomedical imaging technologies. Using numerous endogenous and exogenous contrasts, PAT can provide high-resolution images at scales covering organelles, cells, tissues, organs, small-animal organisms, up to humans, and can reveal tissue’s anatomical, functional, metabolic, and even histologic properties, with molecular and neuronal specificity.

At PI-Lab, we develop PAT technologies with novel and advanced imaging performance, in terms of spatial resolutions, imaging speed, penetration depth, detection sensitivity, and functionality. We are interested with all aspects of PAT technology innovations, including efficient light illumination, high-sensitivity ultrasonic detection, super-resolution PAT, high-speed imaging acquisition, novel PA genetic contrast, and precise image reconstruction. On top of the technological advancements, we are devoted to serve the broad life science and medical communities with matching PAT systems for various research and clinical needs. With its unique contrast mechanism, high scalability, and inherent functional and molecular imaging capabilities, PAT is well suited for a variety of pre-clinical applications, especially for studying tumor angiogenesis, cancer hypoxia, and brain disorders; it is also a promising tool for clinical applications in procedures such as cancer screening, melanoma staging, and endoscopic examination.

Appointments and Affiliations

  • Associate Professor of Biomedical Engineering
  • Associate Professor in Neurology
  • Affiliate, Duke Global Health Institute
  • Faculty Network Member of the Duke Institute for Brain Sciences
  • Member of the Duke Cancer Institute

Contact Information

Education

  • Ph.D. Washington University in St. Louis, 2012

Research Interests

Photoacoustic tomography (PAT) technologies, and translating PAT advances into diagnostic and therapeutic applications, especially in functional brain imaging and early cancer theranostics.

Courses Taught

  • BME 792: Continuation of Graduate Independent Study
  • BME 791: Graduate Independent Study
  • BME 790L: Advanced Topics with the Lab for Graduate Students in Biomedical Engineering
  • BME 590: Special Topics in Biomedical Engineering
  • BME 553: Deep Tissue Optics (GE, IM)
  • BME 494: Projects in Biomedical Engineering (GE)
  • BME 493: Projects in Biomedical Engineering (GE)
  • BME 394: Projects in Biomedical Engineering (GE)
  • BME 303L: Modern Diagnostic Imaging Systems (AC or GE)

In the News

Representative Publications

  • Maharjan, Sushila, Chenshuo Ma, Bibhor Singh, Heemin Kang, Gorka Orive, Junjie Yao, and Yu Shrike Zhang. “Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.” Advanced Drug Delivery Reviews 208 (May 2024): 115237. https://doi.org/10.1016/j.addr.2024.115237.
  • Zhu, Xiaoyi, Qiang Huang, Laiming Jiang, Van-Tu Nguyen, Tri Vu, Garth Devlin, Jabbar Shaima, et al. “Longitudinal intravital imaging of mouse placenta.” Sci Adv 10, no. 12 (March 22, 2024): eadk1278. https://doi.org/10.1126/sciadv.adk1278.
  • Zhang, Wenxu, Yajuan Li, Anthony A. Fung, Zhi Li, Hongje Jang, Honghao Zha, Xiaoping Chen, et al. “Multi-molecular hyperspectral PRM-SRS microscopy.” Nat Commun 15, no. 1 (February 21, 2024): 1599. https://doi.org/10.1038/s41467-024-45576-6.
  • Vu, Tri, Paul Klippel, Aidan J. Canning, Chenshuo Ma, Huijuan Zhang, Ludmila A. Kasatkina, Yuqi Tang, et al. “On the Importance of Low-Frequency Signals in Functional and Molecular Photoacoustic Computed Tomography.” IEEE Trans Med Imaging 43, no. 2 (February 2024): 771–83. https://doi.org/10.1109/TMI.2023.3320668.
  • Vu, Tri, Paul Klippel, Aidan J. Canning, Chenshuo Ma, Huijuan Zhang, Ludmila A. Kasatkina, Yuqi Tang, et al. “On the Importance of Low-Frequency Signals in Functional and Molecular Photoacoustic Computed Tomography.” IEEE Transactions on Medical Imaging 43, no. 2 (February 2024): 771–83. https://doi.org/10.1109/tmi.2023.3320668.
  • Cho, Soon-Woo, Van Tu Nguyen, Anthony DiSpirito, Joseph Yang, Chang-Seok Kim, and Junjie Yao. “Sounding out the dynamics: a concise review of high-speed photoacoustic microscopy.” Journal of Biomedical Optics 29, no. Suppl 1 (January 2024): S11521. https://doi.org/10.1117/1.jbo.29.s1.s11521.
  • Kuang, Xiao, Qiangzhou Rong, Saud Belal, Tri Vu, Alice M. López López, Nanchao Wang, Mehmet Onur Arıcan, et al. “Self-enhancing sono-inks enable deep-penetration acoustic volumetric printing.” Science (New York, N.Y.) 382, no. 6675 (December 2023): 1148–55. https://doi.org/10.1126/science.adi1563.
  • Lee, Youngseop, Qiangzhou Rong, Ki-Hee Song, David A. Czaplewski, Hao F. Zhang, Junjie Yao, and Cheng Sun. “Theoretical and experimental study on the detection limit of the micro-ring resonator based ultrasound point detectors.” Photoacoustics 34 (December 2023): 100574. https://doi.org/10.1016/j.pacs.2023.100574.
  • Xiang, Gaoming, Junqin Chen, Derek Ho, Georgy Sankin, Xuning Zhao, Yangyuanchen Liu, Kevin Wang, John Dolbow, Junjie Yao, and Pei Zhong. “Shock waves generated by toroidal bubble collapse are imperative for kidney stone dusting during Holmium:YAG laser lithotripsy.” Ultrasonics Sonochemistry 101 (December 2023): 106649. https://doi.org/10.1016/j.ultsonch.2023.106649.
  • Park, Hyungjoo, Junjie Yao, and Yun Jing. “A frequency-domain model-based reconstruction method for transcranial photoacoustic imaging: A 2D numerical investigation.” Photoacoustics 33 (October 2023): 100561. https://doi.org/10.1016/j.pacs.2023.100561.
  • Rong, Qiangzhou, Carlos Taboada, Ángela del Águila, Ilaria Merutka, Nishad Jayasundara, Yushun Zeng, Wei Yang, Qifa Zhou, and Junjie Yao. “From Ultraviolet to Near-Infrared: Label-Free Reflection-Mode Hyperspectral Photoacoustic Microscopy for Single-Cell Biochemical Mapping.” GEN Biotechnology 2, no. 5 (October 1, 2023): 435–44. https://doi.org/10.1089/genbio.2023.0035.
  • Yao, Junjie, Chulhong Kim, Michael Kolios, and Song Hu. “Editorial: Breaking the speed limits in photoacoustic microscopy.” Photoacoustics 32 (August 2023): 100541. https://doi.org/10.1016/j.pacs.2023.100541.
  • Kim, Howuk, Bohua Zhang, Huaiyu Wu, Junjie Yao, Chengzhi Shi, and Xiaoning Jiang. “Vortex-ultrasound for microbubble-mediated thrombolysis of retracted clots.” Applied Physics Letters 123, no. 7 (August 2023): 073701. https://doi.org/10.1063/5.0155223.
  • Zhang, Dong, Wei Wang, Xiaoyi Zhu, Ran Li, Wei Liu, Maomao Chen, Tri Vu, et al. “Epinephrine-induced Effects on Cerebral Microcirculation and Oxygenation Dynamics Using Multimodal Monitoring and Functional Photoacoustic Microscopy.” Anesthesiology 139, no. 2 (August 1, 2023): 173–85. https://doi.org/10.1097/ALN.0000000000004592.
  • Ma, Chenshuo, Xiao Kuang, Maomao Chen, Luca Menozzi, Laiming Jiang, Qifa Zhou, Yu Shrike Zhang, and Junjie Yao. “Multiscale photoacoustic tomography using reversibly switchable thermochromics.” Journal of Biomedical Optics 28, no. 8 (August 2023): 082804. https://doi.org/10.1117/1.jbo.28.8.082804.
  • Wu, Huaiyu, Yuqi Tang, Bohua Zhang, Paul Klippel, Yun Jing, Junjie Yao, and Xiaoning Jiang. “Miniaturized Stacked Transducer for Intravascular Sonothrombolysis With Internal-Illumination Photoacoustic Imaging Guidance and Clot Characterization.” IEEE Transactions on Bio-Medical Engineering 70, no. 8 (August 2023): 2279–88. https://doi.org/10.1109/tbme.2023.3240725.
  • Tang, Yuqi, Zhijie Dong, Nanchao Wang, Angela Del Aguila, Natalie Johnston, Tri Vu, Chenshuo Ma, et al. “Non-invasive Deep-Brain Imaging with 3D Integrated Photoacoustic Tomography and Ultrasound Localization Microscopy (3D-PAULM).” ArXiv, July 27, 2023.
  • Menozzi, Luca, Ángela Del Águila, Tri Vu, Chenshuo Ma, Wei Yang, and Junjie Yao. “Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke.” J Vis Exp, no. 196 (June 2, 2023). https://doi.org/10.3791/65319.
  • Veverka, Mitchell, Luca Menozzi, and Junjie Yao. “The sound of blood: photoacoustic imaging in blood analysis.” Medicine in Novel Technology and Devices 18 (June 2023): 100219. https://doi.org/10.1016/j.medntd.2023.100219.
  • Li, Ran, Weina Duan, Dong Zhang, Ulrike Hoffmann, Junjie Yao, Wei Yang, and Huaxin Sheng. “Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation.” J Vis Exp, no. 194 (April 14, 2023). https://doi.org/10.3791/65340.
  • Wang, Ruofan, Jing Zhu, Jun Xia, Junjie Yao, Junhui Shi, and Chiye Li. “Photoacoustic imaging with limited sampling: a review of machine learning approaches.” Biomedical Optics Express 14, no. 4 (April 2023): 1777–99. https://doi.org/10.1364/boe.483081.
  • Wang, Zhuoran, Qiang Li, Brad J. Kolls, Brian Mace, Shu Yu, Xuan Li, Wei Liu, et al. “Sustained overexpression of spliced X-box-binding protein-1 in neurons leads to spontaneous seizures and sudden death in mice.” Commun Biol 6, no. 1 (March 9, 2023): 252. https://doi.org/10.1038/s42003-023-04594-8.
  • Cupil-Garcia, Vanessa, Joy Q. Li, Stephen J. Norton, Ren A. Odion, Pietro Strobbia, Luca Menozzi, Chenshuo Ma, et al. “Plasmonic nanorod probes' journey inside plant cells for in vivo SERS sensing and multimodal imaging.” Nanoscale 15, no. 13 (March 2023): 6396–6407. https://doi.org/10.1039/d2nr06235f.
  • Oliinyk, Olena S., Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Taboada, Huaxin Sheng, Junjie Yao, and Vladislav V. Verkhusha. “Author Correction: Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.” Nat Methods 20, no. 3 (March 2023): 469. https://doi.org/10.1038/s41592-023-01798-y.
  • Yao, J., and V. T. Nguyen. “High-Speed Photoacoustic Microscopy Captures Processes Within Tissue.” Biophotonics International 30, no. 2 (March 1, 2023): 29–34.
  • Xiang, Gaoming, Daiwei Li, Junqin Chen, Arpit Mishra, Georgy Sankin, Xuning Zhao, Yuqi Tang, Kevin Wang, Junjie Yao, and Pei Zhong. “Dissimilar cavitation dynamics and damage patterns produced by parallel fiber alignment to the stone surface in holmium:yttrium aluminum garnet laser lithotripsy.” Physics of Fluids (Woodbury, N.Y. : 1994) 35, no. 3 (March 2023): 033303. https://doi.org/10.1063/5.0139741.
  • Jing, Yun, Mucong Li, Juanjuan Gu, Pei Zhong, and Junjie Yao. “Time-resolved passive cavitation mapping using the transient angular spectrum approach.” In The Journal of the Acoustical Society of America, 153:A268–A268. Acoustical Society of America (ASA), 2023. https://doi.org/10.1121/10.0018806.
  • Menozzi, Luca, Ángela Del Águila, Tri Vu, Chenshuo Ma, Wei Yang, and Junjie Yao. “Three-dimensional non-invasive brain imaging of ischemic stroke by integrated photoacoustic, ultrasound and angiographic tomography (PAUSAT).” Photoacoustics 29 (February 2023): 100444. https://doi.org/10.1016/j.pacs.2022.100444.
  • Tang, Yuqi, Shanshan Tang, Chengwu Huang, Paul Klippel, Chenshuo Ma, Nathan Caso, Shigao Chen, Yun Jing, and Junjie Yao. “High-fidelity deep functional photoacoustic tomography enhanced by virtual point sources.” Photoacoustics 29 (February 2023): 100450. https://doi.org/10.1016/j.pacs.2023.100450.
  • Li, Mucong, Nathan Beaumont, Chenshuo Ma, Juan Rojas, Tri Vu, Max Harlacher, Graeme O’Connell, et al. “Three-Dimensional Deep-Tissue Functional and Molecular Imaging by Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT).” IEEE Trans Med Imaging 41, no. 10 (October 2022): 2704–14. https://doi.org/10.1109/TMI.2022.3168859.
  • Chen, Junqin, Daiwei Li, Wenjun Yu, Zhiteng Ma, Chenhang Li, Gaoming Xiang, Yuan Wu, Junjie Yao, and Pei Zhong. “The Effects of Scanning Speed and Standoff Distance of the Fiber on Dusting Efficiency during Short Pulse Holmium: YAG Laser Lithotripsy.” J Clin Med 11, no. 17 (August 28, 2022). https://doi.org/10.3390/jcm11175048.
  • Zhang, Huijuan, Wei Bo, Depeng Wang, Anthony DiSpirito, Chuqin Huang, Nikhila Nyayapathi, Emily Zheng, et al. “Deep-E: A Fully-Dense Neural Network for Improving the Elevation Resolution in Linear-Array-Based Photoacoustic Tomography.” IEEE Transactions on Medical Imaging 41, no. 5 (May 2022): 1279–88. https://doi.org/10.1109/tmi.2021.3137060.
  • Kasatkina, Ludmila A., Chenshuo Ma, Mikhail E. Matlashov, Tri Vu, Mucong Li, Andrii A. Kaberniuk, Junjie Yao, and Vladislav V. Verkhusha. “Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model.” Nature Communications 13, no. 1 (May 2022): 2813. https://doi.org/10.1038/s41467-022-30547-6.
  • Tang, Yuqi, Huaiyu Wu, Paul Klippel, Bohua Zhang, Hsiao-Ying Shadow Huang, Yun Jing, Xiaoning Jiang, and Junjie Yao. “Deep thrombosis characterization using photoacoustic imaging with intravascular light delivery.” Biomedical Engineering Letters 12, no. 2 (May 2022): 135–45. https://doi.org/10.1007/s13534-022-00216-0.
  • Duan, Tingyang, Xiaorui Peng, Maomao Chen, Dong Zhang, Fei Gao, and Junjie Yao. “Detection of weak optical absorption by optical-resolution photoacoustic microscopy.” Photoacoustics 25 (March 2022): 100335. https://doi.org/10.1016/j.pacs.2022.100335.
  • Dong, Biqin, Junjie Yao, and Xosé Luís Deán-Ben. “Editorial: Advances in Photoacoustic Neuroimaging.” Frontiers in Neuroscience 16 (January 2022): 859515. https://doi.org/10.3389/fnins.2022.859515.
  • Zhang, Dong, Ran Li, Maomao Chen, Tri Vu, Huaxin Sheng, Wei Yang, Ulrike Hoffmann, Jianwen Luo, and Junjie Yao. “Photoacoustic imaging of in vivo hemodynamic responses to sodium nitroprusside.” J Biophotonics 14, no. 7 (July 2021): e202000478. https://doi.org/10.1002/jbio.202000478.
  • Li, Mucong, Juanjuan Gu, Tri Vu, Georgy Sankin, Pei Zhong, Junjie Yao, and Yun Jing. “Time-Resolved Passive Cavitation Mapping Using the Transient Angular Spectrum Approach.” IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control 68, no. 7 (July 2021): 2361–69. https://doi.org/10.1109/tuffc.2021.3062357.
  • Tang, Yuqi, and Junjie Yao. “3D Monte Carlo simulation of light distribution in mouse brain in quantitative photoacoustic computed tomography.” Quantitative Imaging in Medicine and Surgery 11, no. 3 (March 2021): 1046–59. https://doi.org/10.21037/qims-20-815.
  • Li, Mucong, Georgy Sankin, Tri Vu, Junjie Yao, and Pei Zhong. “Tri-modality cavitation mapping in shock wave lithotripsy.” The Journal of the Acoustical Society of America 149, no. 2 (February 2021): 1258. https://doi.org/10.1121/10.0003555.
  • DiSpirito, Anthony, Daiwei Li, Tri Vu, Maomao Chen, Dong Zhang, Jianwen Luo, Roarke Horstmeyer, and Junjie Yao. “Reconstructing Undersampled Photoacoustic Microscopy Images Using Deep Learning.” IEEE Transactions on Medical Imaging 40, no. 2 (February 2021): 562–70. https://doi.org/10.1109/tmi.2020.3031541.
  • III, Anthony DiSpirito, Daiwei Li, Tri Vu, Maomao Chen, Dong Zhang, Jianwen Luo, Roarke Horstmeyer, and Junjie Yao. “Reconstructing Undersampled Photoacoustic Microscopy Images Using Deep Learning.” IEEE Trans. Medical Imaging 40 (2021): 562–70. https://doi.org/10.1109/TMI.2020.3031541.
  • Li, Mucong, Tri Vu, Georgy Sankin, Brenton Winship, Kohldon Boydston, Russell Terry, Pei Zhong, and Junjie Yao. “Internal-Illumination Photoacoustic Tomography Enhanced by a Graded-Scattering Fiber Diffuser.” IEEE Transactions on Medical Imaging 40, no. 1 (January 2021): 346–56. https://doi.org/10.1109/tmi.2020.3027199.
  • Wu, H., Y. Tang, H. Kim, J. Yao, and X. Jiang. “Fiber based laser ultrasound transducer for intravascular thrombolysis with detective photoacoustic imaging.” In IEEE International Ultrasonics Symposium, IUS, Vol. 2020-September, 2020. https://doi.org/10.1109/IUS46767.2020.9251732.
  • Li, M., and J. Yao. “Internal-Illumination Photoacoustic Tomography Enhanced by a Graded-scattering Fiber Diffuser.” In 2020 IEEE Photonics Conference, IPC 2020 - Proceedings, 2020. https://doi.org/10.1109/IPC47351.2020.9252241.
  • Yao, J. “Ultrasfast Wide-field Photoacoustic Microscopy of Small-animal Models.” In 2020 IEEE Photonics Conference, IPC 2020 - Proceedings, 2020. https://doi.org/10.1109/IPC47351.2020.9252208.
  • Vu, Tri, Mucong Li, Hannah Humayun, Yuan Zhou, and Junjie Yao. “A generative adversarial network for artifact removal in photoacoustic computed tomography with a linear-array transducer.” Experimental Biology and Medicine (Maywood, N.J.) 245, no. 7 (April 2020): 597–605. https://doi.org/10.1177/1535370220914285.
  • Li, Yang, Lei Li, Liren Zhu, Konstantin Maslov, Junhui Shi, Peng Hu, En Bo, et al. “Snapshot Photoacoustic Topography Through an Ergodic Relay for High-throughput Imaging of Optical Absorption.” Nature Photonics 14, no. 3 (March 2020): 164–70. https://doi.org/10.1038/s41566-019-0576-2.
  • Li, Mucong, Bangxin Lan, Georgii Sankin, Yuan Zhou, Wei Liu, Jun Xia, Depeng Wang, Gregg Trahey, Pei Zhong, and Junjie Yao. “Simultaneous Photoacoustic Imaging and Cavitation Mapping in Shockwave Lithotripsy.” IEEE Transactions on Medical Imaging 39, no. 2 (February 2020): 468–77. https://doi.org/10.1109/tmi.2019.2928740.
  • Tang, Yuqi, Xuejun Qian, Darrin J. Lee, Qifa Zhou, and Junjie Yao. “From Light to Sound: Photoacoustic and Ultrasound Imaging in Fundamental Research of Alzheimer's Disease.” OBM Neurobiology 4, no. 2 (January 2020). https://doi.org/10.21926/obm.neurobiol.2002056.
  • Onufer, Emily J., Bola Aladegbami, Toru Imai, Kristen Seiler, Adam Bajinting, Cathleen Courtney, Stephanie Sutton, et al. “EGFR in enterocytes & endothelium and HIF1α in enterocytes are dispensable for massive small bowel resection induced angiogenesis.” PloS One 15, no. 9 (January 2020): e0236964. https://doi.org/10.1371/journal.pone.0236964.
  • Vu, Tri, Daniel Razansky, and Junjie Yao. “Listening to tissues with new light: recent technological advances in photoacoustic imaging.” Journal of Optics (2010) 21, no. 10 (October 2019). https://doi.org/10.1088/2040-8986/ab3b1a.
  • Chen, Maomao, Hailey J. Knox, Yuqi Tang, Wei Liu, Liming Nie, Jefferson Chan, and Junjie Yao. “Simultaneous photoacoustic imaging of intravascular and tissue oxygenation.” Optics Letters 44, no. 15 (August 2019): 3773–76. https://doi.org/10.1364/ol.44.003773.
  • Liang, Bingyang, Wei Liu, Qiwei Zhan, Mucong Li, Mingwei Zhuang, Qing H. Liu, and Junjie Yao. “Impacts of the murine skull on high-frequency transcranial photoacoustic brain imaging.” Journal of Biophotonics 12, no. 7 (July 2019): e201800466. https://doi.org/10.1002/jbio.201800466.
  • Humayun, Hannah, and Junjie Yao. “Imaging the aged brain: pertinence and methods.” Quantitative Imaging in Medicine and Surgery 9, no. 5 (May 2019): 842–57. https://doi.org/10.21037/qims.2019.05.06.
  • Zhou, Yuan, Mucong Li, Wei Liu, Georgy Sankin, Jianwen Luo, Pei Zhong, and Junjie Yao. “Thermal Memory Based Photoacoustic Imaging of Temperature.” Optica 6, no. 2 (February 2019): 198–205. https://doi.org/10.1364/optica.6.000198.
  • Lan, Bangxin, Wei Liu, Ya-Chao Wang, Junhui Shi, Yang Li, Song Xu, Huaxin Sheng, et al. “High-speed widefield photoacoustic microscopy of small-animal hemodynamics.” Biomed Opt Express 9, no. 10 (October 1, 2018): 4689–4701. https://doi.org/10.1364/BOE.9.004689.
  • Vienneau, Emelina, Wei Liu, and Junjie Yao. “Dual-view acoustic-resolution photoacoustic microscopy with enhanced resolution isotropy.” Optics Letters 43, no. 18 (September 2018): 4413–16. https://doi.org/10.1364/ol.43.004413.
  • Shi, Junhui, Yuqi Tang, and Junjie Yao. “Advances in super-resolution photoacoustic imaging.” Quantitative Imaging in Medicine and Surgery 8, no. 8 (September 2018): 724–32. https://doi.org/10.21037/qims.2018.09.14.
  • Li, Lei, Anton A. Shemetov, Mikhail Baloban, Peng Hu, Liren Zhu, Daria M. Shcherbakova, Ruiying Zhang, et al. “Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo.” Nature Communications 9, no. 1 (July 2018): 2734. https://doi.org/10.1038/s41467-018-05231-3.
  • Liu, Wei, Daria M. Shcherbakova, Neel Kurupassery, Yang Li, Qifa Zhou, Vladislav V. Verkhusha, and Junjie Yao. “Quad-mode functional and molecular photoacoustic microscopy.” Scientific Reports 8, no. 1 (July 2018): 11123. https://doi.org/10.1038/s41598-018-29249-1.
  • Liu, Wei, Yuan Zhou, Mengran Wang, Lei Li, Emelina Vienneau, Ruimin Chen, Jianwen Luo, et al. “Correcting the limited view in optical-resolution photoacoustic microscopy.” Journal of Biophotonics 11, no. 2 (February 2018). https://doi.org/10.1002/jbio.201700196.
  • Liu, Wei, Bangxin Lan, Leo Hu, Ruimin Chen, Qifa Zhou, and Junjie Yao. “Photoacoustic thermal flowmetry with a single light source.” Journal of Biomedical Optics 22, no. 9 (September 2017): 1–6. https://doi.org/10.1117/1.jbo.22.9.096001.
  • Brunker, Joanna, Junjie Yao, Jan Laufer, and Sarah E. Bohndiek. “Photoacoustic imaging using genetically encoded reporters: a review.” Journal of Biomedical Optics 22, no. 7 (July 2017). https://doi.org/10.1117/1.jbo.22.7.070901.
  • Yao, Junjie. “When pressure meets light: detecting the photoacoustic effect at the origin.” Light, Science & Applications 6, no. 6 (June 2017): e17062. https://doi.org/10.1038/lsa.2017.62.
  • Li, Lei, Liren Zhu, Cheng Ma, Li Lin, Junjie Yao, Lidai Wang, Konstantin Maslov, et al. “Single-impulse Panoramic Photoacoustic Computed Tomography of Small-animal Whole-body Dynamics at High Spatiotemporal Resolution.” Nature Biomedical Engineering 1, no. 5 (January 2017): 0071. https://doi.org/10.1038/s41551-017-0071.
  • Wang, Lihong V., and Junjie Yao. “A practical guide to photoacoustic tomography in the life sciences.” Nature Methods 13, no. 8 (July 2016): 627–38. https://doi.org/10.1038/nmeth.3925.
  • Zhou, Yong, Junjie Yao, and Lihong V. Wang. “Tutorial on photoacoustic tomography.” Journal of Biomedical Optics 21, no. 6 (June 2016): 61007. https://doi.org/10.1117/1.jbo.21.6.061007.
  • Lin, Li, Junjie Yao, Lei Li, and Lihong V. Wang. “In vivo photoacoustic tomography of myoglobin oxygen saturation.” Journal of Biomedical Optics 21, no. 6 (June 2016): 61002. https://doi.org/10.1117/1.jbo.21.6.061002.
  • Hai, Pengfei, Junjie Yao, Guo Li, Chiye Li, and Lihong V. Wang. “Photoacoustic elastography.” Optics Letters 41, no. 4 (February 2016): 725–28. https://doi.org/10.1364/ol.41.000725.
  • Yao, Junjie, Andrii A. Kaberniuk, Lei Li, Daria M. Shcherbakova, Ruiying Zhang, Lidai Wang, Guo Li, Vladislav V. Verkhusha, and Lihong V. Wang. “Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.” Nature Methods 13, no. 1 (January 2016): 67–73. https://doi.org/10.1038/nmeth.3656.
  • Yao, Junjie, Liang Song, and Lihing V. Wang. “Photoacoustic microscopy: superdepth, superresolution, and superb contrast.” IEEE Pulse 6, no. 3 (May 2015): 34–37. https://doi.org/10.1109/mpul.2015.2409100.
  • Yao, Junjie, Lidai Wang, Joon-Mo Yang, Konstantin I. Maslov, Terence T. W. Wong, Lei Li, Chih-Hsien Huang, Jun Zou, and Lihong V. Wang. “High-speed label-free functional photoacoustic microscopy of mouse brain in action.” Nature Methods 12, no. 5 (May 2015): 407–10. https://doi.org/10.1038/nmeth.3336.
  • Xia, Jun, Junjie Yao, and Lihong V. Wang. “Photoacoustic tomography: principles and advances.” Electromagnetic Waves (Cambridge, Mass.) 147 (January 2014): 1–22. https://doi.org/10.2528/pier14032303.
  • Wang, Lidai, Junjie Yao, Konstantin I. Maslov, Wenxin Xing, and Lihong V. Wang. “Ultrasound-heated photoacoustic flowmetry.” Journal of Biomedical Optics 18, no. 11 (November 2013): 117003. https://doi.org/10.1117/1.jbo.18.11.117003.
  • Yao, Junjie, and Lihong V. Wang. “Photoacoustic Microscopy.” Laser & Photonics Reviews 7, no. 5 (September 2013). https://doi.org/10.1002/lpor.201200060.
  • Zhang, Ruiying, Junjie Yao, Konstantin I. Maslov, and Lihong V. Wang. “Structured-illumination photoacoustic Doppler flowmetry of axial flow in homogeneous scattering media.” Applied Physics Letters 103, no. 9 (August 2013): 94101. https://doi.org/10.1063/1.4819735.
  • Zhou, Yong, Junjie Yao, and Lihong V. Wang. “Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth.” Optics Letters 38, no. 14 (July 2013): 2592–95. https://doi.org/10.1364/ol.38.002592.
  • Rowland, Kathryn J., Junjie Yao, Lidai Wang, Christopher R. Erwin, Konstantin I. Maslov, Lihong V. Wang, and Brad W. Warner. “Up-regulation of hypoxia-inducible factor 1 alpha and hemodynamic responses following massive small bowel resection.” Journal of Pediatric Surgery 48, no. 6 (June 2013): 1330–39. https://doi.org/10.1016/j.jpedsurg.2013.03.031.
  • Cai, Xin, Yu Zhang, Li Li, Sung-Wook Choi, Matthew R. MacEwan, Junjie Yao, Chulhong Kim, Younan Xia, and Lihong V. Wang. “Investigation of neovascularization in three-dimensional porous scaffolds in vivo by a combination of multiscale photoacoustic microscopy and optical coherence tomography.” Tissue Engineering. Part C, Methods 19, no. 3 (March 2013): 196–204. https://doi.org/10.1089/ten.tec.2012.0326.
  • Huang, Chih-Hsien, Junjie Yao, Lihong V. Wang, and Jun Zou. “A water-immersible 2-axis scanning mirror microsystem for ultrasound andha photoacoustic microscopic imaging applications.” Microsystem Technologies : Sensors, Actuators, Systems Integration 19, no. 4 (January 2013): 577–82. https://doi.org/10.1007/s00542-012-1660-4.
  • Yao, Junjie, Jun Xia, Konstantin I. Maslov, Mohammadreza Nasiriavanaki, Vassiliy Tsytsarev, Alexei V. Demchenko, and Lihong V. Wang. “Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.” NeuroImage 64 (January 2013): 257–66. https://doi.org/10.1016/j.neuroimage.2012.08.054.
  • Zhang, Chi, Konstantin Maslov, Junjie Yao, and Lihong V. Wang. “In vivo photoacoustic microscopy with 7.6-µm axial resolution using a commercial 125-MHz ultrasonic transducer.” Journal of Biomedical Optics 17, no. 11 (November 2012): 116016. https://doi.org/10.1117/1.jbo.17.11.116016.
  • Yang, Joon-Mo, Ruimin Chen, Christopher Favazza, Junjie Yao, Chiye Li, Zhilin Hu, Qifa Zhou, K Kirk Shung, and Lihong V. Wang. “A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy.” Optics Express 20, no. 21 (October 2012): 23944–53. https://doi.org/10.1364/oe.20.023944.
  • Yao, Junjie, Chih-Hsien Huang, Lidai Wang, Joon-Mo Yang, Liang Gao, Konstantin I. Maslov, Jun Zou, and Lihong V. Wang. “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror.” Journal of Biomedical Optics 17, no. 8 (August 2012): 080505–080501. https://doi.org/10.1117/1.jbo.17.8.080505.
  • Yao, Junjie, Konstantin I. Maslov, and Lihong V. Wang. “In vivo photoacoustic tomography of total blood flow and potential imaging of cancer angiogenesis and hypermetabolism.” Technology in Cancer Research & Treatment 11, no. 4 (August 2012): 301–7. https://doi.org/10.7785/tcrt.2012.500278.
  • Yang, Joon-Mo, Christopher Favazza, Ruimin Chen, Junjie Yao, Xin Cai, Konstantin Maslov, Qifa Zhou, K Kirk Shung, and Lihong V. Wang. “Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo.” Nature Medicine 18, no. 8 (August 2012): 1297–1302. https://doi.org/10.1038/nm.2823.
  • Krumholz, Arie, Lidai Wang, Junjie Yao, and Lihong V. Wang. “Functional photoacoustic microscopy of diabetic vasculature.” Journal of Biomedical Optics 17, no. 6 (June 2012): 060502. https://doi.org/10.1117/1.jbo.17.6.060502.
  • Rowland, Kathryn J., Junjie Yao, Lidai Wang, Christopher R. Erwin, Konstantin I. Maslov, Lihong V. Wang, and Brad W. Warner. “Immediate alterations in intestinal oxygen saturation and blood flow after massive small bowel resection as measured by photoacoustic microscopy.” Journal of Pediatric Surgery 47, no. 6 (June 2012): 1143–49. https://doi.org/10.1016/j.jpedsurg.2012.03.020.
  • Yao, Junjie, Konstantin I. Maslov, Ernest R. Puckett, Kathryn J. Rowland, Brad W. Warner, and Lihong V. Wang. “Double-illumination photoacoustic microscopy.” Optics Letters 37, no. 4 (February 2012): 659–61. https://doi.org/10.1364/ol.37.000659.
  • Filonov, Grigory S., Arie Krumholz, Jun Xia, Junjie Yao, Lihong V. Wang, and Vladislav V. Verkhusha. “Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe.” Angewandte Chemie (International Ed. in English) 51, no. 6 (February 2012): 1448–51. https://doi.org/10.1002/anie.201107026.
  • Tsytsarev, Vassiliy, Konstantin I. Maslov, Junjie Yao, Archana R. Parameswar, Alexei V. Demchenko, and Lihong V. Wang. “In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog.” Journal of Neuroscience Methods 203, no. 1 (January 2012): 136–40. https://doi.org/10.1016/j.jneumeth.2011.09.005.
  • Chatni, Muhammad Rameez, Junjie Yao, Amos Danielli, Christopher P. Favazza, Konstantin I. Maslov, and Lihong V. Wang. “Functional photoacoustic microscopy of pH.” Journal of Biomedical Optics 16, no. 10 (October 2011): 100503. https://doi.org/10.1117/1.3644495.
  • Yao, Junjie, and Lihong V. Wang. “Photoacoustic tomography: fundamentals, advances and prospects.” Contrast Media & Molecular Imaging 6, no. 5 (September 2011): 332–45. https://doi.org/10.1002/cmmi.443.
  • Oladipupo, Sunday, Song Hu, Joanna Kovalski, Junjie Yao, Andrea Santeford, Rebecca E. Sohn, Ralph Shohet, Konstantin Maslov, Lihong V. Wang, and Jeffrey M. Arbeit. “VEGF is essential for hypoxia-inducible factor-mediated neovascularization but dispensable for endothelial sprouting.” Proceedings of the National Academy of Sciences of the United States of America 108, no. 32 (August 2011): 13264–69. https://doi.org/10.1073/pnas.1101321108.
  • Krumholz, Arie, Sarah J. Vanvickle-Chavez, Junjie Yao, Timothy P. Fleming, William E. Gillanders, and Lihong V. Wang. “Photoacoustic microscopy of tyrosinase reporter gene in vivo.” Journal of Biomedical Optics 16, no. 8 (August 2011): 080503. https://doi.org/10.1117/1.3606568.
  • Yao, Junjie, Konstantin I. Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang. “Label-free oxygen-metabolic photoacoustic microscopy in vivo.” Journal of Biomedical Optics 16, no. 7 (July 2011): 076003. https://doi.org/10.1117/1.3594786.
  • Tsytsarev, Vassiliy, Song Hu, Junjie Yao, Konstantin Maslov, Dennis L. Barbour, and Lihong V. Wang. “Photoacoustic microscopy of microvascular responses to cortical electrical stimulation.” Journal of Biomedical Optics 16, no. 7 (July 2011): 076002. https://doi.org/10.1117/1.3594785.
  • Oladipupo, Sunday S., Song Hu, Andrea C. Santeford, Junjie Yao, Joanna R. Kovalski, Ralph V. Shohet, Konstantin Maslov, Lihong V. Wang, and Jeffrey M. Arbeit. “Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.” Blood 117, no. 15 (April 2011): 4142–53. https://doi.org/10.1182/blood-2010-09-307538.
  • Kim, Chulhong, Hyon-Min Song, Xin Cai, Junjie Yao, Alexander Wei, and Lihong V. Wang. “In vivo photoacoustic mapping of lymphatic systems with plasmon-resonant nanostars.” Journal of Materials Chemistry 21, no. 9 (January 2011): 2841–44. https://doi.org/10.1039/c0jm04194g.
  • Wang, Lidai, Konstantin Maslov, Junjie Yao, Bin Rao, and Lihong V. Wang. “Fast voice-coil scanning optical-resolution photoacoustic microscopy.” Optics Letters 36, no. 2 (January 2011): 139–41. https://doi.org/10.1364/ol.36.000139.
  • Yao, Junjie, Konstantin I. Maslov, Yunfei Shi, Larry A. Taber, and Lihong V. Wang. “In vivo photoacoustic imaging of transverse blood flow by using Doppler broadening of bandwidth.” Optics Letters 35, no. 9 (May 2010): 1419–21. https://doi.org/10.1364/ol.35.001419.
  • Yao, Junjie, and Lihong V. Wang. “Transverse flow imaging based on photoacoustic Doppler bandwidth broadening.” Journal of Biomedical Optics 15, no. 2 (March 2010): 021304. https://doi.org/10.1117/1.3339953.
  • Yao, Junjie, Konstantin Maslov, Song Hu, and Lihong V. Wang. “Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo.” Journal of Biomedical Optics 14, no. 5 (September 2009): 054049. https://doi.org/10.1117/1.3251044.
  • Song, X. L., G. Hu, J. J. Yao, and J. Bai. “Three-dimensional reconstruction for fluorescence tomography using cylinder phantoms.” Ruan Jian Xue Bao/Journal of Software 20, no. 5 (May 1, 2009): 1089–95. https://doi.org/10.3724/SP.J.1001.2009.03560.
  • Yao, J. J., G. Hu, and J. Bai. “Modeling and validation of light propagation in free-space for non-contact near-infrared fluorescent tomography.” Hongwai Yu Haomibo Xuebao/Journal of Infrared and Millimeter Waves 27, no. 5 (October 1, 2008).
  • Yao, J. J., G. Hu, S. H. Yue, and J. Bai. “A 3D-surface torso reconstruction method used in fluorescence molecular tomography of small animals.” Chinese Journal of Biomedical Engineering 27, no. 3 (June 1, 2008): 360–65.
  • Hu, G., J. Yao, and J. Bai. “Full-angle optical imaging of near-infrared fluorescent probes implanted in small animals.” Progress in Natural Science 18, no. 6 (January 1, 2008): 707–11. https://doi.org/10.1016/j.pnsc.2007.11.021.