Fan Yuan

Research Themes

Biomechanics & Mechanobiology, Drug & Gene Delivery

Research Interests

Principles and mechanisms of delivery for drugs, genes, and vaccines; pharmacokinetics; electrotransfer or electroporation technology; and tumor pathophysiology.

Bio

Dr. Yuan has extensive experiences in analysis of therapeutic agent transport in mammalian cells, tissues, and organs, and development of effective strategies, design principles, and new technologies that can be used to facilitate the transport. The goal of his research is to improve delivery of therapeutic agents to their targets, which is crucial in treatment and prevention of diseases. He has published >100 scientific papers in peer-reviewed journals, and a textbook on transport analysis in biological systems that has been used to teach undergraduate and graduate courses in many universities.

Education

• B.S. Peking University (China), 1983
• M.S. Peking University (China), 1985
• Ph.D. City University of New York, 1990

Positions

• Professor of Biomedical Engineering
• Professor in Ophthalmology
• Member of the Duke Cancer Institute

Awards, Honors, and Distinctions

• Fellow. American Institute for Medical and Biological Engineering. 2007
• CAREER Award. National Science Foundation. 2000
• SPORE in Breast Cancer Career Development Award. Duke University Medical Center. 1996
• IPM Innovative Instrumentation Award. The Microcirculatory Society. 1994
• IPM Innovative Instrumentation Award. The Microcirculatory Society. 1993

Courses Taught

• EGR 391: Projects in Engineering
• BME 791: Graduate Independent Study
• BME 789: Internship in Biomedical Engineering
• BME 577: Drug Delivery (GE, BB, MC)
• BME 530: Introduction to Tissue Biomechanics (GE, BB)

Publications

• Wang C, Yuan F. A comprehensive comparison of DNA and RNA vaccines. Advanced drug delivery reviews. 2024 Jul;210:115340.
• Wang C, Chang C-C, Chi J-T, Yuan F. Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2. Pharmaceutics. 2024 Mar 29;16(4).
• Wang C, Macintyre AN, Oguin TH, McCarthy KR, Moody MA, Yuan F. Spatiotemporal Control of Immune Responses with Nucleic Acid Cocktail Vaccine. Advanced Therapeutics. 2024 Jan 1;
• Sylvers J, Wang Y, Yuan F. Nuclear Entry of DNA and Transgene Expression in Dividing and Non-dividing Cells. Cellular and molecular bioengineering. 2023 Dec;16(5–6):459–74.
• Wang C, Karlsson A, Oguin TH, Macintyre AN, Sempowski GD, McCarthy KR, et al. Transient inhibition of lysosomal functions potentiates nucleic acid vaccines. Proc Natl Acad Sci U S A. 2023 Oct 31;120(44):e2306465120.
• Wang Y, Chang C-C, Yuan F. Copy number of naked DNA delivered into nucleus of mammalian cells by electrotransfection. Bioelectrochemistry (Amsterdam, Netherlands). 2023 Oct;153:108491.
• Wu F, Wang H, Sun C, Yuan F, Xie Z, Mikhaylov R, et al. Power-controlled acoustofluidic manipulation of microparticles. Ultrasonics. 2023 Sep;134:107087.
• Wang H, Yuan F, Xie Z, Sun C, Wu F, Mikhaylov R, et al. Modelling hybrid acoustofluidic devices for enhancing Nano- and Micro-Particle manipulation in microfluidics. Applied Acoustics. 2023 Mar 30;205.
• Jin Z, Yuan F. A Double-Permeability Poroelasticity Model for Fluid Transport in a Biological Tissue. Transport in Porous Media. 2023 Mar 1;147(1):169–95.
• Wang Y, Wang C, Sylvers J, Segura T, Yuan F. Nanoenhancer for improving naked DNA electrotransfection In vivo. Frontiers in bioengineering and biotechnology. 2023 Jan;11:1181795.
• Wu F, Shen MH, Yang J, Wang H, Mikhaylov R, Clayton A, et al. An Enhanced Tilted-Angle Acoustofluidic Chip for Cancer Cell Manipulation. IEEE Electron Device Letters. 2021 Apr 1;42(4):577–80.
• Wang L, Chang C-C, Sylvers J, Yuan F. A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells. Bioelectrochemistry (Amsterdam, Netherlands). 2021 Apr;138:107731.
• Sun C, Wu F, Fu Y, Wallis DJ, Mikhaylov R, Yuan F, et al. Thin film Gallium nitride (GaN) based acoustofluidic Tweezer: Modelling and microparticle manipulation. Ultrasonics. 2020 Dec;108:106202.
• Huang J, Sachdeva M, Xu E, Robinson TJ, Luo L, Ma Y, et al. The Long Noncoding RNA NEAT1 Promotes Sarcoma Metastasis by Regulating RNA Splicing Pathways. Mol Cancer Res. 2020 Oct;18(10):1534–44.
• Wang C, Chang C-C, Wang L, Yuan F. Inhibition of Caspases Improves Non-Viral T Cell Receptor Editing. Cancers. 2020 Sep;12(9):E2603.
• Wang Y, Chang C-C, Wang L, Yuan F. Enhancing Cell Viability and Efficiency of Plasmid DNA Electrotransfer Through Reducing Plasma Membrane Permeabilization. Bioelectricity. 2020 Sep;2(3):251–7.
• Sun C, Wu F, Wallis DJ, Shen MH, Yuan F, Yang J, et al. Gallium Nitride: A Versatile Compound Semiconductor as Novel Piezoelectric Film for Acoustic Tweezer in Manipulation of Cancer Cells. IEEE Transactions on Electron Devices. 2020 Aug 1;67(8):3355–61.
• Mao M, Chang C-C, Pickar-Oliver A, Cervia LD, Wang L, Ji J, et al. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020 Aug;4(8):e2000059.
• Mikhaylov R, Wu F, Wang H, Clayton A, Sun C, Xie Z, et al. Correction: Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB. Lab on a chip. 2020 Aug;20(17):3278.
• Mikhaylov R, Wu F, Wang H, Clayton A, Sun C, Xie Z, et al. Development and characterisation of acoustofluidic devices using detachable electrodes made from PCB. Lab on a chip. 2020 May;20(10):1807–14.
• Wang C, Chang C-C, Yuan F. Inhibition of Caspase-3 Improves Electrotransfer Efficiency and Cell Viability. In: MOLECULAR THERAPY. 2020. p. 473–473.
• Wang L, Miller SE, Yuan F. Ultrastructural Analysis of Vesicular Transport in Electrotransfection. Microsc Microanal. 2018 Oct;24(5):553–63.
• Cervia LD, Yuan F. Current Progress in Electrotransfection as a Nonviral Method for Gene Delivery. Molecular pharmaceutics. 2018 Sep;15(9):3617–24.
• Tao K, Fan Z, Sun L, Makam P, Tian Z, Ruegsegger M, et al. Quantum confined peptide assemblies with tunable visible to near-infrared spectral range. Nature communications. 2018 Aug;9(1):3217.
• Cervia LD, Chang C-C, Wang L, Mao M, Yuan F. Enhancing Electrotransfection Efficiency through Improvement in Nuclear Entry of Plasmid DNA. Molecular therapy Nucleic acids. 2018 Jun;11:263–71.
• Kaakati R, Chang C, Li F, Liu X, Yuan F, Li C. 1213 Exosome-mediated RNAi delivery for melanoma therapy. In: Journal of Investigative Dermatology. Elsevier BV; 2018. p. S206–S206.
• Cervia LD, Chang C-C, Wang L, Mao M, Yuan F. Enhancing Electrotransfection Efficiency Through Improvement in Nuclear Entry of Plasmid DNA. In: BIOPHYSICAL JOURNAL. CELL PRESS; 2018. p. 542A-542A.
• Yuan F. Physiological Barriers to Drug Transport. In: Molecular, Cellular, and Tissue Engineering. 2018. p. 83-1-83–19.
• Lei Y, Han H, Yuan F, Javeed A, Zhao Y. The brain interstitial system: Anatomy, modeling, in vivo measurement, and applications. Progress in neurobiology. 2017 Oct;157:230–46.
• Chang J, Huang J, Li L, Liu Z, Yuan F. Stiffness characterization of anisotropic trabecular meshwork. Journal of biomechanics. 2017 Aug;61:144–50.
• Mao M, Wang L, Chang C-C, Rothenberg KE, Huang J, Wang Y, et al. Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection. Mol Ther. 2017 Mar 1;25(3):803–15.
• Cervia LD, Chang C-C, Wang L, Yuan F. Distinct Effects of Endosomal Escape and Endosomal Trafficking on Gene Delivery via Electrotransfection. In: Biophysical Journal. Elsevier BV; 2017. p. 473a-473a.
• Cervia LD, Chang C-C, Wang L, Yuan F. Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection. PloS one. 2017 Jan;12(2):e0171699.
• Cervia LD, Wang L, Chang C, Mao M, Yuan F. Nuclear Envelope As A Physical Barrier In Electrotransfection. In: MOLECULAR BIOLOGY OF THE CELL. AMER SOC CELL BIOLOGY; 2017.
• Chang C-C, Mao M, Liu Y, Wu M, Vo-Dinh T, Yuan F. Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes. Cellular and molecular bioengineering. 2016 Dec;9(4):538–45.
• Li G, Mukherjee D, Navarro I, Ashpole NE, Sherwood JM, Chang J, et al. Visualization of conventional outflow tissue responses to netarsudil in living mouse eyes. Eur J Pharmacol. 2016 Sep 15;787:20–31.
• Huang J, Wang L, Xiong C, Yuan F. Elastic hydrogel as a sensor for detection of mechanical stress generated by single cells grown in three-dimensional environment. Biomaterials. 2016 Aug;98:103–12.
• Yuan F, Schieber AT, Camras LJ, Harasymowycz PJ, Herndon LW, Allingham RR. Mathematical Modeling of Outflow Facility Increase With Trabecular Meshwork Bypass and Schlemm Canal Dilation. J Glaucoma. 2016 Apr;25(4):355–64.
• Liang J, Yuan F, Gonzalez P, Stamer W. Signaling Pathways that mediate endoMT of Human Trabecular meshwork cells exposed to Cyclic Mechanical Stress. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. ASSOC RESEARCH VISION OPHTHALMOLOGY INC; 2015.
• Huang J, Camras LJ, Yuan F. Mechanical analysis of rat trabecular meshwork. Soft matter. 2015 Apr;11(14):2857–65.
• Lee TJ, Wong J, Bae S, Lee AJ, Lopatkin A, Yuan F, et al. A power-law dependence of bacterial invasion on mammalian host receptors. PLoS computational biology. 2015 Apr;11(4):e1004203.
• Cheung TM, Yan JB, Fu JJ, Huang J, Yuan F, Truskey GA. Endothelial Cell Senescence Increases Traction Forces due to Age-Associated Changes in the Glycocalyx and SIRT1. Cellular and molecular bioengineering. 2015 Mar;8(1):63–75.
• Yuan Y, Huang J, Fang J, Yuan F, Xiong C. A self-adaptive sampling digital image correlation algorithm for accurate displacement measurement. Optics and Lasers in Engineering. 2015 Feb 1;65:57–63.
• Novak MT, Yuan F, Reichert WM. Macrophage embedded fibrin gels: an in vitro platform for assessing inflammation effects on implantable glucose sensors. Biomaterials. 2014 Dec;35(36):9563–72.
• Liang J, Navarro ID, Gonzalez P, Yuan F, Stamer WD. Mechanical and cytokine stimulation of smooth muscle actin expression in human trabecular meshwork cells. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. ASSOC RESEARCH VISION OPHTHALMOLOGY INC; 2014.
• Camras LJ, Stamer WD, Epstein D, Gonzalez P, Yuan F. Circumferential tensile stiffness of glaucomatous trabecular meshwork. Invest Ophthalmol Vis Sci. 2014 Feb 10;55(2):814–23.
• Liu C, Li C-Y, Yuan F. Mathematical modeling of the Phoenix Rising pathway. PLoS Comput Biol. 2014 Feb;10(2):e1003461.
• Yuan Y, Huang J, Peng X, Xiong C, Fang J, Yuan F. Accurate displacement measurement via a self-adaptive digital image correlation method based on a weighted ZNSSD criterion. Optics and Lasers in Engineering. 2014 Jan 1;52(1):75–85.
• Chang C-C, Wu M, Yuan F. Role of specific endocytic pathways in electrotransfection of cells. Molecular therapy Methods & clinical development. 2014 Jan;1:14058.
• Li G, Farsiu S, Qiu J, Dixon A, Song C, McKinnon SJ, et al. Disease progression in iridocorneal angle tissues of BMP2-induced ocular hypertensive mice with optical coherence tomography. Mol Vis. 2014;20:1695–709.
• Novak MT, Yuan F, Reichert WM. Predicting glucose sensor behavior in blood using transport modeling: relative impacts of protein biofouling and cellular metabolic effects. Journal of diabetes science and technology. 2013 Nov;7(6):1547–60.
• Camras L, Stamer WD, Epstein D, Gonzalez P, Yuan F. Circumferential Trabecular Meshwork Stiffness in Glaucomatous Eyes. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. ASSOC RESEARCH VISION OPHTHALMOLOGY INC; 2013.
• Yuan F, Camras L. Local Stiffness of Rat Trabecular Meshwork. In: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. ASSOC RESEARCH VISION OPHTHALMOLOGY INC; 2013.
• Huang J, Pan X, Peng X, Yuan Y, Xiong C, Fang J, et al. Digital Image Correlation with Self-Adaptive Gaussian Windows. Experimental Mechanics. 2013 Mar 1;53(3):505–12.
• McGuire S, Yuan F. Improving interstitial transport of macromolecules through reduction in cell volume fraction in tumor tissues. Nanomedicine : nanotechnology, biology, and medicine. 2012 Oct;8(7):1088–95.
• Camras LJ, Yuan F, Fan S, Samuelson TW, Ahmed IK, Schieber AT, et al. A novel Schlemm's Canal scaffold increases outflow facility in a human anterior segment perfusion model. Investigative ophthalmology & visual science. 2012 Sep;53(10):6115–21.
• Camras LJ, Stamer WD, Epstein D, Gonzalez P, Yuan F. Differential effects of trabecular meshwork stiffness on outflow facility in normal human and porcine eyes. Invest Ophthalmol Vis Sci. 2012 Aug 9;53(9):5242–50.
• Rivera S, Yuan F. Critical issues in delivery of RNAi therapeutics in vivo. Current pharmaceutical biotechnology. 2012 Jun;13(7):1279–91.
• Elliott NT, Yuan F. A microfluidic system for investigation of extravascular transport and cellular uptake of drugs in tumors. Biotechnology and bioengineering. 2012 May;109(5):1326–35.
• Luna C, Li G, Huang J, Qiu J, Wu J, Yuan F, et al. Regulation of trabecular meshwork cell contraction and intraocular pressure by miR-200c. PLoS One. 2012;7(12):e51688.
• Long M, Yuan F. Special issue: Experiments and modeling in microand nano-biomechanics. Cellular and Molecular Bioengineering. 2011 Sep 1;4(3):325–6.
• Elliott N, Lee T, You L, Yuan F. Proliferation behavior of E. coli in a three-dimensional in vitro tumor model. Integrative biology : quantitative biosciences from nano to macro. 2011 Jun;3(6):696–705.
• Wu M, Yuan F. Membrane binding of plasmid DNA and endocytic pathways are involved in electrotransfection of mammalian cells. PloS one. 2011 Jan;6(6):e20923.
• Elliott NT, Yuan F. A review of three-dimensional in vitro tissue models for drug discovery and transport studies. Journal of pharmaceutical sciences. 2011 Jan;100(1):59–74.
• Henshaw J, Mossop B, Yuan F. Enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution. Cancer gene therapy. 2011 Jan;18(1):26–33.
• Lin C-W, Gonzalez P, Yuan F. Cellular pharmacokinetic and pharmacodynamic analyses of ethacrynic acid: Implications in topical drug delivery in the eye. Mol Vis. 2011;17:2507–15.
• Novak MT, Yuan F, Reichert WM. Modeling the relative impact of capsular tissue effects on implanted glucose sensor time lag and signal attenuation. Analytical and bioanalytical chemistry. 2010 Oct;398(4):1695–705.
• Lin C-W, Yuan F. Numerical simulations of ethacrynic acid transport from precorneal region to trabecular meshwork. Annals of biomedical engineering. 2010 Mar;38(3):935–44.
• Yarmolenko PS, Zhao Y, Landon C, Spasojevic I, Yuan F, Needham D, et al. Comparative effects of thermosensitive doxorubicin-containing liposomes and hyperthermia in human and murine tumours. Int J Hyperthermia. 2010;26(5):485–98.
• Novak MT, Yuan F, Reichert WM. Modeling the Relative Effects of Biofouling, Fibrous Encapsulation and Microvessel Density on Implanted Glucose Sensor Performance. Biophysical Journal. 2010 Jan;98(3):407a-407a.
• Chen Q, Krol A, Wright A, Needham D, Dewhirst MW, Yuan F. Tumor microvascular permeability is a key determinant for antivascular effects of doxorubicin encapsulated in a temperature sensitive liposome. Int J Hyperthermia. 2008 Sep;24(6):475–82.
• Henshaw J, Mossop B, Yuan F. Relaxin treatment of solid tumors: effects on electric field-mediated gene delivery. Molecular cancer therapeutics. 2008 Aug;7(8):2566–73.
• Henshaw JW, Yuan F. Field distribution and DNA transport in solid tumors during electric field-mediated gene delivery. Journal of pharmaceutical sciences. 2008 Feb;97(2):691–711.
• Tong S, Yuan F. Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: II. Numerical simulations. Microvascular research. 2008 Jan;75(1):16–24.
• Yuan F, Huang A. Effects of transmural fluid exchange on tumor blood flow. BIORHEOLOGY. 2008 Jan 1;45(1–2):39–39.
• Zaharoff DA, Henshaw JW, Mossop B, Yuan F. Mechanistic analysis of electroporation-induced cellular uptake of macromolecules. Experimental biology and medicine (Maywood, NJ). 2008 Jan;233(1):94–105.
• Tong S, Yuan F. Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: I. Experimental characterizations. Microvascular research. 2008 Jan;75(1):10–5.
• Henshaw JW, Zaharoff DA, Mossop BJ, Yuan F. Electric field-mediated transport of plasmid DNA in tumor interstitium in vivo. Bioelectrochemistry (Amsterdam, Netherlands). 2007 Nov;71(2):233–42.
• Mossop BJ, Barr RC, Henshaw JW, Yuan F. Electric fields around and within single cells during electroporation-a model study. Annals of biomedical engineering. 2007 Jul;35(7):1264–75.
• Lin C-W, Wang Y, Challa P, Epstein DL, Yuan F. Transscleral diffusion of ethacrynic acid and sodium fluorescein. Mol Vis. 2007 Feb 22;13:243–51.
• Norton LW, Yuan F, Reichert WM. Glucose recovery with bare and hydrogel-coated microdialysis probes: experiment and simulation of temporal effects. Analytical chemistry. 2007 Jan;79(2):445–52.
• Reza F, Chandran K, Feltz M, Heinz A, Josephs E, O’Brien P, et al. Engineering novel synthetic biological systems. IET Synthetic Biology. 2007;1:48–52.
• Needham D, Wright A, Tong J, Dewhirst MW, Ponce A, Yuan F. Mapping engineering onto biology at the nanoscale: nature's encapsulation technologies as bioinspiration for nano-scale anti-tumor drug delivery. In: Nanomedicine: Nanotechnology, Biology and Medicine. Elsevier BV; 2006. p. 292–3.
• Mossop BJ, Barr RC, Henshaw JW, Zaharoff DA, Yuan F. Electric fields in tumors exposed to external voltage sources: implication for electric field-mediated drug and gene delivery. Annals of biomedical engineering. 2006 Oct;34(10):1564–72.
• Henshaw JW, Zaharoff DA, Mossop BJ, Yuan F. A single molecule detection method for understanding mechanisms of electric field-mediated interstitial transport of genes. Bioelectrochemistry (Amsterdam, Netherlands). 2006 Oct;69(2):248–53.
• Shan S, Flowers C, Peltz CD, Sweet H, Maurer N, Kwon E-JG, et al. Preferential extravasation and accumulation of liposomal vincristine in tumor comparing to normal tissue enhances antitumor activity. Cancer Chemother Pharmacol. 2006 Aug;58(2):245–55.
• McGuire S, Zaharoff D, Yuan F. Nonlinear dependence of hydraulic conductivity on tissue deformation during intratumoral infusion. Annals of biomedical engineering. 2006 Jul;34(7):1173–81.
• Ponce AM, Vujaskovic Z, Yuan F, Needham D, Dewhirst MW. Hyperthermia mediated liposomal drug delivery. Int J Hyperthermia. 2006 May;22(3):205–13.
• McGuire S, Zaharoff D, Yuan F. Effects of tumor tissue collagen content on the flow rate during intratumoral infusion. In: CANCER RESEARCH. AMER ASSOC CANCER RESEARCH; 2006.
• Dreher MR, Liu W, Michelich CR, Dewhirst MW, Yuan F, Chilkoti A. Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. J Natl Cancer Inst. 2006 Mar 1;98(5):335–44.
• Wang Y, Wang H, Li C-Y, Yuan F. Effects of rate, volume, and dose of intratumoral infusion on virus dissemination in local gene delivery. Mol Cancer Ther. 2006 Feb;5(2):362–6.
• Wang Y, Yuan F. Delivery of viral vectors to tumor cells: extracellular transport, systemic distribution, and strategies for improvement. Annals of biomedical engineering. 2006 Jan;34(1):114–27.
• Tong S, Chen Q, Shan S-Q, Dewhirst MW, Yuan F. Quantitative comparison of the inhibitory effects of GW5638 and tamoxifen on angiogenesis in the cornea pocket assay. Angiogenesis. 2006;9(2):53–8.
• Mossop BJ, Henshaw JW, Zaharoff DA, Yuan F. Ex vivo and in vivo mapping of electric fields in tumor tissues. Proceedings of the 2005 Summer Bioengineering Conference. 2005 Dec 1;2005:197–8.
• Lin CW, Yuan F. Numerical simulations of transcorneal tranport of ethacrynic acid. Proceedings of the 2005 Summer Bioengineering Conference. 2005 Dec 1;2005:289–90.
• Henshaw JW, Zaharoff DA, Mossop BJ, Yuan F. In vivo electric field-mediated transport of plasmid DNA in tumor interstitium. Proceedings of the 2005 Summer Bioengineering Conference. 2005 Dec 1;2005:149–50.
• Wang Y, Chen Q, Yuan F. Alginate encapsulation is a highly reproducible method for tumor cell implantation in dorsal skinfold chambers. BioTechniques. 2005 Dec;39(6):834–9.
• Wang Y, Liu S, Li C-Y, Yuan F. A novel method for viral gene delivery in solid tumors. Cancer Res. 2005 Sep 1;65(17):7541–5.
• Wang Y, Yang Z, Liu S, Kon T, Krol A, Li C-Y, et al. Characterisation of systemic dissemination of nonreplicating adenoviral vectors from tumours in local gene delivery. Br J Cancer. 2005 Apr 25;92(8):1414–20.
• Chen Q, Tong S, Dewhirst MW, Yuan F. Targeting tumor microvessels using doxorubicin encapsulated in a novel thermosensitive liposome. Mol Cancer Ther. 2004 Oct;3(10):1311–7.
• Mossop BJ, Barr RC, Zaharoff DA, Yuan F. Electric fields within cells as a function of membrane resistivity--a model study. IEEE transactions on nanobioscience. 2004 Sep;3(3):225–31.
• Wang Y, Challa P, Epstein DL, Yuan F. Controlled release of ethacrynic acid from poly(lactide-co-glycolide) films for glaucoma treatment. Biomaterials. 2004 Aug;25(18):4279–85.
• Zaharoff DA, Yuan F. Effects of pulse strength and pulse duration on in vitro DNA electromobility. Bioelectrochemistry (Amsterdam, Netherlands). 2004 Apr;62(1):37–45.
• Olbrich KC, Yuan F. Simulation of angiogenesis in engineered tissues: Effects of VEGF binding to matrix components. In: FASEB JOURNAL. FEDERATION AMER SOC EXP BIOL; 2004. p. A372–A372.
• Wang Y, Li C-Y, Yuan F. Systemic virus dissemination during local gene delivery in solid tumors and its control with an alginate solution. Conf Proc IEEE Eng Med Biol Soc. 2004;2004:3524–6.
• Wang Y, Hu JK, Krol A, Li Y-P, Li C-Y, Yuan F. Systemic dissemination of viral vectors during intratumoral injection. Mol Cancer Ther. 2003 Nov;2(11):1233–42.
• Sampson JH, Akabani G, Archer GE, Bigner DD, Berger MS, Friedman AH, et al. Progress report of a Phase I study of the intracerebral microinfusion of a recombinant chimeric protein composed of transforming growth factor (TGF)-alpha and a mutated form of the Pseudomonas exotoxin termed PE-38 (TP-38) for the treatment of malignant brain tumors. J Neurooncol. 2003 Oct;65(1):27–35.
• Zaharoff DA, Yuan F. Transmembrane transport of marker molecules and cell membrane kinetics during electropermeabilization. In: MOLECULAR THERAPY. ACADEMIC PRESS INC ELSEVIER SCIENCE; 2003. p. S64–S64.
• Olbrich KC, Tong S, Yuan F. Simulation modeling of angiogenesis in engineered tissues: effects of growth factor supplementation. In: FASEB JOURNAL. FEDERATION AMER SOC EXP BIOL; 2003. p. A147–A147.
• Lockhart AC, Braun RD, Yu D, Ross JR, Dewhirst MW, Humphrey JS, et al. Reduction of wound angiogenesis in patients treated with BMS-275291, a broad spectrum matrix metalloproteinase inhibitor. Clin Cancer Res. 2003 Feb;9(2):586–93.
• Krol A, Dewhirst MW, Yuan F. Effects of cell damage and glycosaminoglycan degradation on available extravascular space of different dextrans in a rat fibrosarcoma. Int J Hyperthermia. 2003;19(2):154–64.
• Lockhart AC, Braun RD, Yu D, Ross JR, Dewhirst MW, Klitzman B, et al. A clinical model of dermal wound angiogenesis. Wound Repair Regen. 2003;11(4):306–13.
• McGuire SM, Yuan F. Effects of tissue stretching or cell shrinkage on penetration depth of macromolecules in a rat fibrosarcoma. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:516–7.
• Lin CW, Wang Y, Yuan F. Transscleral diffusion of ethacrynic acid and sodium fluorescein. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:515.
• Zaharoff DA, Yuan F. Effects of electric pulse strength and pulse duration on plasmid DNA electromobility. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:546–7.
• Wang Y, Hu JK, Krol A, Li YP, Li CY, Yuan F. Reducing systemic toxicity in local tumor gene therapy using an alginate solution. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:565.
• Zaharoff DA, Barr RC, Li C-Y, Yuan F. Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery. Gene Ther. 2002 Oct;9(19):1286–90.
• Tong S, Yuan F. An equivalent length model of microdialysis sampling. Journal of pharmaceutical and biomedical analysis. 2002 Apr;28(2):269–78.
• Dewhirst MW, Shan S, Cao Y, Moeller B, Yuan F, Li C-Y. Intravital fluorescence facilitates measurement of multiple physiologic functions and gene expression in tumors of live animals. Dis Markers. 2002;18(5–6):293–311.
• Yuan F, Krol A, Tong S. Available space and extracellular transport of macromolecules: effects of pore size and connectedness. Annals of biomedical engineering. 2001 Dec;29(12):1150–8.
• McGuire S, Yuan F. Quantitative analysis of intratumoral infusion of color molecules. American journal of physiology Heart and circulatory physiology. 2001 Aug;281(2):H715–21.
• Lohr F, Lo DY, Zaharoff DA, Hu K, Zhang X, Li Y, et al. Effective tumor therapy with plasmid-encoded cytokines combined with in vivo electroporation. Cancer Res. 2001 Apr 15;61(8):3281–4.
• Tong S, Yuan F. Numerical simulations of angiogenesis in the cornea. Microvascular research. 2001 Jan;61(1):14–27.
• Zhang XY, Luck J, Dewhirst MW, Yuan F. Interstitial hydraulic conductivity in a fibrosarcoma. Am J Physiol Heart Circ Physiol. 2000 Dec;279(6):H2726–34.
• McGuire S, Zhang XY, Dewhirst M, Yuan F. New technique for measurement of distribution volume of color molecules in solid tumors. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
• Krol A, Yuan F, Dewhirst M. Available volume fraction of macromolecules in solid tumors. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
• Zaharoff D, Barr R, Li CY, Yuan F. Mobility of plasmid DNA subject to pulsed electric fields. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
• Yuan F, Zaharoff D, Zhang XY, Lohr F, Dewhirst MW, Li CY. Delivery of plasmid DNA through intratumoral infusion and electroporation. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 2000 Dec 1;48:173–6.
• Tong S, Yuan F. Numerical simulations of angiogenesis in the cornea. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
• Dellian M, Yuan F, Trubetskoy VS, Torchilin VP, Jain RK. Vascular permeability in a human tumour xenograft: molecular charge dependence. British journal of cancer. 2000 May;82(9):1513–8.
• Lockhart AC, Braun RD, Dewhirst MW, Humphrey JS, Yuan F, Grichnick J, et al. Wound angiogenesis inhibition as a marker for anti-angiogenic efficacy. In: FASEB JOURNAL. FEDERATION AMER SOC EXP BIOL; 2000. p. A35–A35.
• Tong S, Yuan F. Numerical simulation of angiogenesis in the cornea. FASEB JOURNAL. 2000 Mar 15;14(4):A35–A35.
• Krol A, Nagaraj S, Dewhirst M, Yuan F. Available volume fraction of macromolecules in tumor tissues. FASEB JOURNAL. 2000 Mar 15;14(4):A167–A167.
• McGuire S, Zhang X-Y, Dewhirst M, Yuan F. New technique for measurement of distribution volume of color molecules in solid tumors. Annals of Biomedical Engineering. 2000;28(SUPPL 1):25.
• Zaharoff D, Barr R, Li C-Y, Yuan F. Mobility of plasmid DNA subject to pulsed electric fields. Annals of Biomedical Engineering. 2000;28(SUPPL 1):25.
• Tong S, Yuan F. Numerical simulations of angiogenesis in the cornea. Annals of Biomedical Engineering. 2000;28(SUPPL 1):77.
• Yuan F, Zaharoff D, Zhang XY, Lohr F, Dewhirst MW, Li CY. Delivery of plasmid DNA through intratumoral infusion and electroporation. In: ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE). 2000. p. 125–8.
• Yuan F, Zhang X, Dewhirst MW, Luck JA. Pressure and temperature-dependence of the hydraulic conductivity in a fibrosarcoma. In: Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999. p. 91.
• Krol A, Maresca J, Dewhirst MW, Yuan F. Available volume fraction of macromolecules in the extravascular space of a fibrosarcoma: implications for drug delivery. Cancer Res. 1999 Aug 15;59(16):4136–41.
• Monsky WL, Fukumura D, Gohongi T, Ancukiewcz M, Weich HA, Torchilin VP, et al. Augmentation of transvascular transport of macromolecules and nanoparticles in tumors using vascular endothelial growth factor. Cancer research. 1999 Aug;59(16):4129–35.
• Jain RK, Safabakhsh N, Sckell A, Chen Y, Jiang P, Benjamin L, et al. Endothelial cell death, angiogenesis, and microvascular function after castration in an androgen-dependent tumor: role of vascular endothelial growth factor. Proceedings of the National Academy of Sciences of the United States of America. 1998 Sep;95(18):10820–5.
• Yuan F. Transvascular drug delivery in solid tumors. Seminars in radiation oncology. 1998 Jul;8(3):164–75.
• Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, et al. Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proceedings of the National Academy of Sciences of the United States of America. 1998 Apr;95(8):4607–12.
• Monsky WL, Hobbs SK, Yuan F, Torchilin VP, Jain RK. Modulation of intratumoral drug delivery by the tumor microenvironment and vascular endothelial growth factor. Proceedings of the Controlled Release Society. 1998 Jan 1;(25):207–8.
• Jain RK, Schlenger K, Höckel M, Yuan F. Quantitative angiogenesis assays: progress and problems. Nature medicine. 1997 Nov;3(11):1203–8.
• Fukumura D, Yuan F, Monsky WL, Chen Y, Jain RK. Effect of host microenvironment on the microcirculation of human colon adenocarcinoma. The American journal of pathology. 1997 Sep;151(3):679–88.
• Nozue M, Lee I, Yuan F, Teicher BA, Brizel DM, Dewhirst MW, et al. Interlaboratory variation in oxygen tension measurement by Eppendorf "Histograph" and comparison with hypoxic marker. J Surg Oncol. 1997 Sep;66(1):30–8.
• Yuan F. Stress is good and bad for tumors. Nature biotechnology. 1997 Aug;15(8):722–3.
• Berk DA, Yuan F, Leunig M, Jain RK. Direct in vivo measurement of targeted binding in a human tumor xenograft. Proceedings of the National Academy of Sciences of the United States of America. 1997 Mar;94(5):1785–90.
• Fukumura D, Yuan F, Endo M, Jain RK. Role of nitric oxide in tumor microcirculation. Blood flow, vascular permeability, and leukocyte-endothelial interactions. The American journal of pathology. 1997 Feb;150(2):713–25.
• Helmlinger G, Yuan F, Dellian M, Jain RK. Interstitial pH and pO2 gradients in solid tumors in vivo: high-resolution measurements reveal a lack of correlation. Nature medicine. 1997 Feb;3(2):177–82.
• Leunig M, Yuan F, Gerweck LE, Jain RK. Effect of basic fibroblast growth factor on angiogenesis and growth of isografted bone: quantitative in vitro-in vivo analysis in mice. International journal of microcirculation, clinical and experimental. 1997 Jan;17(1):1–9.
• Yuan F, Chen Y, Dellian M, Safabakhsh N, Ferrara N, Jain RK. Time-dependent vascular regression and permeability changes in established human tumor xenografts induced by an anti-vascular endothelial growth factor/vascular permeability factor antibody. Proceedings of the National Academy of Sciences of the United States of America. 1996 Dec;93(25):14765–70.
• Lichtenbeld HC, Yuan F, Michel CC, Jain RK. Perfusion of single tumor microvessels: application to vascular permeability measurement. Microcirculation (New York, NY : 1994). 1996 Dec;3(4):349–57.
• Dellian M, Helmlinger G, Yuan F, Jain RK. Fluorescence ratio imaging of interstitial pH in solid tumours: effect of glucose on spatial and temporal gradients. British journal of cancer. 1996 Oct;74(8):1206–15.
• Leunig M, Yuan F, Berk DA, Gerweck LE, Jain RK. Heating or freezing bone. Effects on angiogenesis induction and growth potential in mice. Acta orthopaedica Scandinavica. 1996 Aug;67(4):383–8.
• Dellian M, Witwer BP, Salehi HA, Yuan F, Jain RK. Quantitation and physiological characterization of angiogenic vessels in mice: effect of basic fibroblast growth factor, vascular endothelial growth factor/vascular permeability factor, and host microenvironment. The American journal of pathology. 1996 Jul;149(1):59–71.
• Yamada S, Mayadas TN, Yuan F, Wagner DD, Hynes RO, Melder RJ, et al. Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin. Blood. 1995 Nov;86(9):3487–92.
• Yuan F, Dellian M, Fukumura D, Leunig M, Berk DA, Torchilin VP, et al. Vascular permeability in a human tumor xenograft: molecular size dependence and cutoff size. Cancer research. 1995 Sep;55(17):3752–6.
• Leunig M, Yuan F, Gerweck LE, Berk DA, Jain RK. Quantitative analysis of angiogenesis and growth of bone: effect of indomethacin exposure in a combined in vitro-in vivo approach. Research in experimental medicine Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie. 1995 Jan;195(5):275–88.
• Leu AJ, Berk DA, Yuan F, Jain RK. Flow velocity in the superficial lymphatic network of the mouse tail. The American journal of physiology. 1994 Oct;267(4 Pt 2):H1507–13.
• Yuan F, Salehi HA, Boucher Y, Vasthare US, Tuma RF, Jain RK. Vascular permeability and microcirculation of gliomas and mammary carcinomas transplanted in rat and mouse cranial windows. Cancer research. 1994 Sep;54(17):4564–8.
• Leunig M, Yuan F, Berk DA, Gerweck LE, Jain RK. Angiogenesis and growth of isografted bone: quantitative in vivo assay in nude mice. Laboratory investigation; a journal of technical methods and pathology. 1994 Aug;71(2):300–7.
• Yuan F, Leunig M, Huang SK, Berk DA, Papahadjopoulos D, Jain RK. Microvascular permeability and interstitial penetration of sterically stabilized (stealth) liposomes in a human tumor xenograft. Cancer research. 1994 Jul;54(13):3352–6.
• Torres Filho IP, Leunig M, Yuan F, Intaglietta M, Jain RK. Noninvasive measurement of microvascular and interstitial oxygen profiles in a human tumor in SCID mice. Proceedings of the National Academy of Sciences of the United States of America. 1994 Mar;91(6):2081–5.
• Berk DA, Yuan F, Leunig M, Jain RK. Fluorescence photobleaching with spatial Fourier analysis: measurement of diffusion in light-scattering media. Biophysical journal. 1993 Dec;65(6):2428–36.
• Yuan F, Leunig M, Berk DA, Jain RK. Microvascular permeability of albumin, vascular surface area, and vascular volume measured in human adenocarcinoma LS174T using dorsal chamber in SCID mice. Microvascular research. 1993 May;45(3):269–89.
• Jain RK, Baxter LT, Boucher Y, Leunig M, Yuan F. Transport of fluid and macromolecules in tumors. In: American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD. 1992. p. 103–4.
• Leunig M, Yuan F, Menger MD, Boucher Y, Goetz AE, Messmer K, et al. Angiogenesis, microvascular architecture, microhemodynamics, and interstitial fluid pressure during early growth of human adenocarcinoma LS174T in SCID mice. Cancer research. 1992 Dec;52(23):6553–60.
• Baxter LT, Yuan F, Jain RK. Pharmacokinetic analysis of the perivascular distribution of bifunctional antibodies and haptens: comparison with experimental data. Cancer research. 1992 Oct;52(20):5838–44.
• Yuan F, Chien S, Weinbaum S. A new view of convective-diffusive transport processes in the arterial intima. Journal of biomechanical engineering. 1991 Aug;113(3):314–29.
• Yuan F, Baxter LT, Jain RK. Pharmacokinetic analysis of two-step approaches using bifunctional and enzyme-conjugated antibodies. Cancer research. 1991 Jun;51(12):3119–30.
• Yuan F, Weinbaum S, Pfeffer R, Chien S. A mathematical model for the receptor mediated cellular regulation of the low density lipoprotein metabolism. Journal of biomechanical engineering. 1991 Feb;113(1):1–10.
• Yuan F, Chien S, Weinbaum S. New view of convective-diffusive processes in the arterial intima. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 1990 Dec 1;17:33–5.
• Fan Y, Wang-yi W. The displacement wave theory of blood vessel. Applied Mathematics and Mechanics. 1989 Jun;10(6):487–93.