Professor of Biomedical Engineering
Bursac's research interests include pluripotent stem cell therapies for heart and muscle disease. Cardiac and skeletal muscle tissue engineering. Cardiac electrophysiology and arrhythmias. Genetic modifications of stem and somatic cells. Micropatterning of proteins and hydrogels. Organ-on-chip technologies.
The focus of my research is application of stem cells and tissue engineering methodologies in experimental in vitro studies and cell and tissue replacement therapies. Micropatterning of extracellular matrix proteins or protein hydrogels and engineering of synthetic scaffolds are used to build stem cell-derived cardiac and skeletal muscle tissues that replicate the structure-function relationships present in healthy and diseased muscle. These systems are used to separate and systematically study the roles of structural and genetic factors that contribute cardiac and skeletal muscle function and disease at multiple organizational levels (from single cell to 3-dimensional tissue). Optical recordings with voltage and calcium sensitive dyes in synthetic tissues allow us to analyze and optimize normal electrical function as well as study complicated spatio-temporal changes in electrical activity encountered in cardiac arrhythmias and fibrillation. Contractile force measurements allow us to explore factors that would optimize mechanical function of engineered tissues. Examples of the current research projects include: 1) design of co-cultures made of cardiac and different types of stem cells to model and study cell and tissue therapies for cardiac infarction and arrhythmias, 2) local and global gene manipulation in cultures of cardiac and other cell types, 3) engineering of vascularized cardiac and skeletal muscle tissue constructs with controllable structure and function, 4) implantation of stem cell-derived cardiac tissue patches in animal models of cardiac infarction, and 5) design of synthetic excitable tissues for experimental studies and novel cell therapies.
Appointments and Affiliations
- Professor of Biomedical Engineering
- Associate Professor in Medicine
- Member of the Duke Cancer Institute
- Office Phone: (919) 660-5510
- Email Address: email@example.com
- Ph.D. Boston University, 2000
- B.S.E. University of Belgrade, 1994
Research InterestsBursac's research interests include embryonic and adult stem cell therapies for heart and muscle disease. Cardiac and skeletal muscle tissue engineering. Cardiac electrophysiology and arrhythmias. Genetic modifications of stem and somatic cells. Micropatterning of proteins and hydrogels.
Tissue Repair, Tissue Engineering
- BME 301L: Bioelectricity (AC or GE)
- BME 394: Projects in Biomedical Engineering (GE)
- BME 493: Projects in Biomedical Engineering (GE)
- BME 494: Projects in Biomedical Engineering (GE)
- BME 507: Cardiovascular System Engineering, Disease and Therapy (GE, BB, EL)
- BME 578: Quantitative Cell and Tissue Engineering (GE, BB, MC)
- BME 590: Advanced Topics in Biomedical Engineering
- ECE 391: Undergraduate Research in Electrical and Computer Engineering
- GLHLTH 393: Research Independent Study in Global Health
- NEUROSCI 301L: Bioelectricity (AC or GE)
- Juhas, M; Ye, J; Bursac, N, Design, evaluation, and application of engineered skeletal muscle., Methods, vol 99 (2016), pp. 81-90 [10.1016/j.ymeth.2015.10.002] [abs].
- Zhang, H; Sun, AY; Kim, JJ; Graham, V; Finch, EA; Nepliouev, I; Zhao, G; Li, T; Lederer, WJ; Stiber, JA; Pitt, GS; Bursac, N; Rosenberg, PB, STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node., Proceedings of the National Academy of Sciences of USA, vol 112 no. 41 (2015), pp. E5618-E5627 [abs].
- Juhas, M; Wang, J; Ye, J; Shadrin, I; Bursac, N, Engineering Regenerative Skeletal Muscle Tissues, Tissue Engineering, Part A, vol 21 (2015), pp. S310-S310 [abs].
- Madden, L; Jackman, C; Wang, J; Kraus, W; Truskey, G; Bursac, N, Novel In Vitro Exercise Model of Engineered Human Skeletal Muscle, Tissue Engineering, Part A, vol 21 (2015), pp. S46-S46 [abs].
- Madden, L; Koeberl, D; Bursac, N, BIOENGINEERED HUMAN MUSCLE FOR PHYSIOLOGICAL STUDIES AND DISEASE MODELING, Molecular Genetics and Metabolism, vol 114 no. 3 (2015), pp. 304-305 [abs].